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Addgene
Showing: 2141 - 2160 of 13889 results
  1. pIS0

    Plasmid
    #12178
    Purpose
    3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production
    Depositor
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pAd/CMV/PBX1-IRES-nEBFP2

    Plasmid
    #29755
    Depositor
    Insert
    Pre-B-cell leukemia transcription factor 1 (PBX1 Human)
    Use
    Tags
    EBFP2, IRES, and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    No mutations; EBFP2 is nuclear localized; PBX1 an…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pAd/WT1-IRES-nAmCyan

    Plasmid
    #29756
    Depositor
    Insert
    Wilms tumor 1 (WT1 Human)
    Use
    Tags
    AmCyan, IRES, and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    No mutations; AmCyan is nuclear localized; WT1 an…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pJK569

    Plasmid
    #72455
    Purpose
    Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene
    Depositor
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~51 silent substitutions
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  5. pJK573

    Plasmid
    #72456
    Purpose
    Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene
    Depositor
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~57 silent substitutions
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  6. pCW57-MCS1-P2A-MCS2 (Neo)

    Plasmid
    #89180
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Depositor
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Availability
    Academic Institutions and Nonprofits only
  7. TALEN_C63

    Plasmid
    #40788
    Purpose
    Golden Gate Compatible TALEN Construct for site specific modification of genome, +63 C Terminus, Codon Optimized FokI
    Depositor
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  8. OmpA3-PNGaseF-TEV-His6

    Plasmid
    #114274
    Purpose
    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.
    Depositor
    Insert
    Peptide: N-glycosidase F
    Use
    Tags
    OmpA3 leader sequence (for periplasmic localisati…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  9. G-SP140-8

    Plasmid
    #166520
    Purpose
    scFv of a human scaffold targeting Nuclear body protein SP140. Antigen coverage aa 688-862 of 867, Bromodomain
    Depositor
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
Showing: 2141 - 2160 of 13889 results