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Plasmid#40545DepositorInsertLRRK2
UseBaculovirusTags6xHisExpressionMutationWTPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-TEV protease
Plasmid#165509PurposeUsed for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/GDepositorInsertTEV protease
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-iLOVf
Plasmid#63723PurposeE coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteinsDepositorTypeEmpty backboneUseTagsTEE, iLOV, 6xHis, thrombin cleavage siteExpressionBacterialMutationPromoterT7Available sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP prescission LIC cloning vector (HMPKS)
Plasmid#29721DepositorTypeEmpty backboneUseTagsHis6-MBP-prescissionExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
C113m
Plasmid#121012PurposeMoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.DepositorInsertCas9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C95m
Plasmid#121001PurposeMoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.DepositorInsertT7 RNA polymerase (Andy please check!)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
371-A
Plasmid#97026PurposeyORFDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP-Nucleus-7
Plasmid#55265PurposeLocalization: Nuclear Localization, Excitation: 399, Emission: 456DepositorInsertSV40 T-Antigen Nuclear Localization Signal (NLS)
UseTagsmTagBFP-NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-TopBP1-mCer
Plasmid#211414Purposemammalian expression of human TopBP1 C-terminally fused to mCeruleanDepositorInsertTopBP1 (TOPBP1 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ (M)-HT-NLS
Plasmid#82518PurposeLentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.DepositorInsertNuclear localizaition sequence
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOpen-K12polLF
Plasmid#165511PurposeDNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.DepositorInsertDNA Polymerase I, Large (Klenow) Fragment
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-aASIC1
Plasmid#32795DepositorInsertASIC1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RecQL4-mCer
Plasmid#211402Purposemammalian expression of human RecQL4 C-terminally fused to mCeruleanDepositorInsertRecQL4 (RECQL4 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
371-U
Plasmid#97036PurposeMYFQSNA-yORFDepositorTypeEmpty backboneUseTagsMYFQSNAExpressionBacterialMutationPromoterT7Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX
Plasmid#52421PurposeExpresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherryDepositorInsertFusion NLS-EGFP-P2A-Cherry-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACYC-LIC+
Plasmid#62312PurposeE. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage siteDepositorTypeEmpty backboneUseTags6xHis and thrombin protease cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Nucleus-7
Plasmid#54206PurposeLocalization: Nucleus, Excitation: 487, Emission: 509DepositorInsertSV40 T-Antigen Nuclear Localization Signal (NLS)
UseTagsmEmerald-NucleusExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-Nucleus-7
Plasmid#55249PurposeLocalization: Nuclear Localization, Excitation: 383, Emission: 448DepositorInsertSV40 T-Antigen Nuclear Localization Signal (NLS)
UseTagsEBFP2-NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
U25m
Plasmid#121027PurposeMoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.DepositorInsertRiboJ + UTR1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJO-7
Plasmid#31992DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only