pCR2.1_HBB_A
(Plasmid #87848)

• Purpose: pCR2.1-based plasmid holding a 328-bp fragment containing the exon-1-exon-2 splice border of aberrant HBB[IVSI-110(G>A)] human β-globin mRNA
• Depositing Lab: Marina Kleanthous
• Publication: Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii: ijms21186671. doi: 10.3390/ijms21186671.

Backbone

• Vector backbone: pCR2.1-TOPO
• Backbone manufacturer: Invitrogen (Life Technologies)
• Backbone size w/o insert (bp): 3930
• Total vector size (bp): 4256
• Vector type: Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Homo sapiens hemoglobin subunit beta (HBB), Aberrant cDNA fragment (Exon1/19nt mispliced Intron1 + Exon2)
• Species: H. sapiens (human)
• Insert Size (bp): 329
• Mutation: HBB(IVSI-110) β-thalassaemia misplicing mutation (HGVS name: HBB:c.93-21G>A)
• GenBank ID: NM_000518.4
• Entrez Gene: HBB (a.k.a. CD113t-C, ECYT6, beta-globin)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: M13 Reverse Primer
• 3′ sequencing primer: M13 Forward Primer

Resource Information

• Supplemental Documents:
  • pCR(R)2.1-TOPO IVSI 110 cl2.gb
• A portion of this plasmid was derived from a plasmid made by: Petros Patsali

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry