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Plasmid#58306PurposeGeneGuard genomic cassette: for constructing ĪdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.DepositorInsertGenomic cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15TV-L-AldA WT
Plasmid#187976PurposeExpresses human aldolase ADepositorInsertAldA WT (ALDOA Human)
UseTagsHexahistagExpressionBacterialMutationPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-EPN-01*
Plasmid#80423PurposeFor expression of EPN-01* in E. coliDepositorInsertEPN-01*
UseTagsMycExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQmod2S-GG
Plasmid#191346PurposeClostridium expression vector (pBP1 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJKR-L-acuR
Plasmid#62568PurposeAcrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
GLI-3 bs-2
Plasmid#16420DepositorInsertGLI3 (GLI3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mCherry2-L
Plasmid#104003PurposePlasmid encoding mCherry2-LDepositorInsertmCherry2-L
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGEE NNLA IIKEFā¦ Vā¦PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAT9679-sgRNA-BFP
Plasmid#162988PurposesgRNA cloning plasmidDepositorInsertexchangeable cassette
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-STEPtag
Plasmid#159373PurposeExpresses STEPtag in E. coliDepositorInsertSTEPtag
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTA-attP
Plasmid#18939DepositorInsertphiC31 attP
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDF0044 CjcCas7-11 full locus with synthetic array
Plasmid#172502PurposeEncodes the CjcCas7-11 full locus for bacterial expressionDepositorInsertCjcCas7-11 full locus with synthetic array
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>m2a(silent)-srt-his
Plasmid#124807PurposeHDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.DepositorInsertMurine IgG2a (Fc silent)
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationL234A/L235A/N297APromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2
Plasmid#145370PurposeGateway destination vector for cell-free and mamalian expression with N-term 8xHis-tag and mCherry tagDepositorTypeEmpty backboneUseTagsHis and mCherryExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYC.Taq.1Amb
Plasmid#102794PurposeExpresses Taq DNA polymerases with a premature stop codonDepositorInsertTaq DNA polymerase
UseTagsExpressionBacterialMutationW168Stop; *(see below)PromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB402-er-mCherry
Plasmid#186288PurposeBinary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)DepositorInserter-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBBA57-TEV-His12
Plasmid#137033Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66270.1 (BB_A57 Borrelia burgdorferi B31)
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length BBA57, contains signal pepā¦PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-gp78c/2767
Plasmid#11429DepositorInsertgp78c (AMFR Human)
UseTagsGSTExpressionBacterialMutationn/aPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSiM4
Plasmid#199383PurposeEncodes dual cistronic membrane localized jGCaMP7f and mScarlet-I optimized for C. elegans expressionDepositorInsertjGCaMP6f::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
UseTagsExpressionBacterialMutationno mutations, except added subcellular localizatiā¦PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-Ubl
Plasmid#78712PurposeExpresses Sacsin Ubl- domain (2-85) in fusion with GST (bacterial)DepositorInsertHuman Sacsin 2-85
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only