pET15TV-L-AldA WT
(Plasmid #187976)

• Purpose: Expresses human aldolase A
• Depositing Lab: Audrey Lamb
• Publication: Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi: 10.1002/pro.4222. Epub 2021 Nov 12.

Backbone

• Vector backbone: pET15TV-L
• Backbone manufacturer: Cheryl Arrowsmith
• Backbone size w/o insert (bp): 7746
• Total vector size (bp): 8838
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AldA WT
• Alt name: human aldolase A
• Species: H. sapiens (human)
• Insert Size (bp): 1092
• GenBank ID: CAG46678.1
• Entrez Gene: ALDOA (a.k.a. ALDA, GSD12, HEL-S-87p)
• Promoter: T7
• Tag / Fusion Protein:
  • Hexahistag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: ATG CGT CCG GCG TAG A
• 3′ sequencing primer: TAG TTA TTG CTC AGC GGT GG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene was codon optimized and synthesized into pET15TV-L by Genscript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry