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Addgene
Showing: 1121 - 1140 of 147311 results

  1. pJK641

    Plasmid
    #72468
    Purpose
    Encodes Escherichia coli evgA (Plac noncoding)
    Depositor
    T. J. Kappock
    Insert
    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Availability
    Academic Institutions and Nonprofits only
    72468-plasmid-map-sequence-id-134015

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  2. pJK646

    Plasmid
    #72469
    Purpose
    Encodes Escherichia coli evgA (Plac coding)
    Depositor
    T. J. Kappock
    Insert
    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Availability
    Academic Institutions and Nonprofits only
    72469-plasmid-map-sequence-id-134016

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  3. pJK652

    Plasmid
    #72470
    Purpose
    Produces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)
    Depositor
    T. J. Kappock
    Insert
    acetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated histidine-233 to glycine
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72470-plasmid-map-sequence-id-134018

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  4. pJK217

    Plasmid
    #72471
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase with N-terminal His6 tag (H6AaAarA)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72471-plasmid-map-sequence-id-135051

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  5. pJK317

    Plasmid
    #72472
    Purpose
    puc118 modified to contain ribosome binding site
    Depositor
    T. J. Kappock
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Availability
    Academic Institutions and Nonprofits only
    72472-plasmid-map-sequence-id-140449

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  6. pJK635

    Plasmid
    #72473
    Purpose
    pET28a modified to contain TEV protease fusion tag instead of T7 tag
    Depositor
    T. J. Kappock
    Type
    Empty backbone
    Use
    Tags
    His6 and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72473-plasmid-map-sequence-id-132656

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  7. pJK383

    Plasmid
    #72474
    Purpose
    Produces Thermoplasma acidophilum citrate synthase with Asp317>Gly mutant (TpCS-D317G)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-17 to phenylalanine, tryptopha…
    Promoter
    recA
    Availability
    Academic Institutions and Nonprofits only
    72474-plasmid-map-sequence-id-140430

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  8. pJK436

    Plasmid
    #72477
    Purpose
    Produces Thermoplasma acidophilum citrate synthase
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72477-plasmid-map-sequence-id-134619

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  9. pJK439

    Plasmid
    #72478
    Purpose
    Produces Thermoplasma acidophilum citrate synthase pseudo-dimer
    Depositor
    T. J. Kappock
    Insert
    citrate synthase (artificial dimer)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted NSTCLVPRGSPV sequence between C- and N-t…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72478-plasmid-map-sequence-id-134617

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  10. pJK520

    Plasmid
    #72479
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic gene
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    Use
    Gene synthesis
    Tags
    His6
    Expression
    Mutation
    synthetic gene
    Promoter
    n/a and none
    Availability
    Academic Institutions and Nonprofits only
    72479-plasmid-map-sequence-id-132532

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  12. RIP7-RLuc-YFP-pBS

    Plasmid
    #72482
    Purpose
    Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals
    Depositor
    Vincent Poitout
    Article
    Ghislain et al Endocrinology. 2015 Dec 15:en20151550.
    Inserts
    rat ins2 gene promoter (Ins2 Rat)
    luciferase
    eYFP
    Use
    Luciferase
    Tags
    eYFP and renilla luciferase
    Expression
    Mammalian
    Mutation
    eYFP varient
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    72482-plasmid-map-sequence-id-136970

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  13. pCDH-EF1s

    Plasmid
    #72484
    Purpose
    Express gene of interest under truncated EF-1 promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    72484-plasmid-map-sequence-id-134994

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  14. pCDH-EF1-Luc2-P2A-copGFP

    Plasmid
    #72485
    Purpose
    Express Luc2 and copGFP
    Depositor
    Kazuhiro Oka
    Insert
    Luc2-P2A-copGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1
    Availability
    Industry, Academic Institutions, and Nonprofits
    72485-plasmid-map-sequence-id-132335

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  15. pCDH-EF1-Luc2-P2A-tdTomato

    Plasmid
    #72486
    Purpose
    Express Luc2 and tdTomato under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    Luc2-P2A-tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Availability
    Academic Institutions and Nonprofits only
    72486-plasmid-map-sequence-id-418875

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  16. CFP-GIV-F1685A

    Plasmid
    #72489
    Purpose
    Mammalian expression of The CFP-tagged GIV-F1685A biosensors
    Depositor
    Pradipta Ghosh
    Insert
    GIV/Girdin (CCDC88A Human)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    F1685A
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  20. pJK522

    Plasmid
    #72510
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Availability
    Academic Institutions and Nonprofits only
    72510-plasmid-map-sequence-id-132535

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Showing: 1121 - 1140 of 147311 results