169,804 results
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Plasmid#211257PurposeMammalian expression of full-length human WDR62 isoform 4. N-terminal HiBiT tag.DepositorInsertWDR62:M1-H1518 (WDR62 Human)
TagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationisoform 4. L850S natural VAR_031299. Q1305L n…PromoterCMVAvailable SinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_CHAF1B:1-559
Plasmid#211144PurposeMammalian expression of full-length human CHAF1B. N-terminal HiBiT tag.DepositorInsertCHAF1B:M1-P559 (CHAF1B Human)
TagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_RFWD3:425-774
Plasmid#210884PurposeInsect expression of human RFWD3 fragment S425 to E774. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertRFWD3:S425-E774 (RFWD3 Human)
TagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBT-RP2.2
Plasmid#189842PurposeIntron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 2.DepositorTypeEmpty backboneUseCre/LoxTagsGFP GM2 variant and mRFPPromoterbeta ActinAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
hIgG1-F1-Fc
Plasmid#194597PurposeMammalian expression of F1 nanobody upstream of human IgG1; F1 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertF1-Fc (S )
ExpressionMammalianAvailable SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTK_Sox10_enh_89_Citrine
Plasmid#130587Purposeenhancer reporterDepositorInsertSox10_enh_89
UseEnhancer reporterPromoterthymidine kinase minimal promoterAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS3_K227/230R_Keima
Plasmid#127146DepositorAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Cucumis sativus
Plasmid#75357PurposeNt His-tag Cucumis sativus Sacsin 1-1292DepositorInsertSacsin Cucumis sativus 1-1292
TagsHis-TagExpressionBacterialAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1dn
Plasmid#174824PurposeMammalian expression of human MLH1dn (codon optimized)DepositorInserthuman MLH1dn (codon optimized)
ExpressionMammalianMutationdeletion of residues 754-756PromoterEF1aAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-miniTurbo-NLS-Blast
Plasmid#232347PurposeLentiviral control vector expressing miniTurboNLS without dCas9DepositorInsertminiTurbo
UseLentiviralTags3xHA and 3xSV40 NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG
Plasmid#70183PurposeInducible expression of guide RNA with fluorescent GFP reporterDepositorInsertH1-Tet-sgrna cassette
UseCRISPR and LentiviralExpressionMammalianAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
gRNA_AAVS1-T2
Plasmid#41818PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T2
UseCRISPRExpressionMammalianAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 pegRNA1 with trimmed attP
Plasmid#222346PurposepegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA1
UseCRISPRAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 pegRNA2 with trimmed attP
Plasmid#222347PurposepegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA2
UseCRISPRAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1
Plasmid#20061PurposeBacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formationDepositorInsertMSP1D1
Tags7-His tagExpressionBacterialMutationdeletion mutant (1-11) of MSP1; His-tag on N-term…Available SinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1
Plasmid#187120Purposemammalian cell expression of FLAG-HUWE1DepositorAvailable SinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Human sgRNA library Brunello in lentiCRISPRv2 (Lentiviral Prep)
Viral Prep#73179-LVPurposeReady-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.DepositorAvailable SinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEcgRNA
Plasmid#166581PurposeCRISPR-Cas9-assisted genome editing in Escherichia coliDepositorInsertccdB
ExpressionBacterialAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)
Plasmid#71814PurposeExpresses high specificity SpCas9. Px330-like plasmid.DepositorHas ServiceCloning Grade DNAInsertenhanced specificity Cas9 (1.1)
UseCRISPRTags3xFLAGExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-mCherry (AAV9)
Viral Prep#50459-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only