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pSQT1601

Plasmid

#53369

Purpose

human codon optimized Csy4 and FokI-dCas9 expression plasmid

Depositor

Keith Joung

Publication

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hCsy4-T2A-NLS-hFokI-dCas9-NLS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pSQT1601

Plasmid

#53369

Purpose

human codon optimized Csy4 and FokI-dCas9 expression plasmid

Depositor

Keith Joung

Publication

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hCsy4-T2A-NLS-hFokI-dCas9-NLS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pMRX-IP-GFP-LC3-RFP

Plasmid

#84573

Purpose

Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Publication

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Available since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

38 Articles

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pMRX-IP-GFP-LC3-RFP

Plasmid

#84573

Purpose

Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Publication

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Available since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

38 Articles

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pAC154-dual-dCas9VP160-sgExpression

Plasmid

#48240

Purpose

Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP160

Expression

Mammalian

Mutation

D10A H840A

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pAC154-dual-dCas9VP160-sgExpression

Plasmid

#48240

Purpose

Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP160

Expression

Mammalian

Mutation

D10A H840A

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

Enlarge
GFP-Nucleolin

Plasmid

#28176

Depositor

Michael Kastan

Publication

Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nucleolin (NCL Human)

Tags

GFP

Expression

Mammalian

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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GFP-Nucleolin

Plasmid

#28176

Depositor

Michael Kastan

Publication

Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nucleolin (NCL Human)

Tags

GFP

Expression

Mammalian

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pGL3 BRE Luciferase

Plasmid

#45126

Depositor

Martine Roussel, Peter ten Dijke

Publication

Korchynskyi et al J Biol Chem. 2002 Feb 15;277(7):4883-91.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Id1-BRE

Use

Luciferase; Bmp/smad transcriptional reporter

Expression

Mammalian

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pGL3 BRE Luciferase

Plasmid

#45126

Depositor

Martine Roussel, Peter ten Dijke

Publication

Korchynskyi et al J Biol Chem. 2002 Feb 15;277(7):4883-91.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Id1-BRE

Use

Luciferase; Bmp/smad transcriptional reporter

Expression

Mammalian

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pENTR1A no ccDB (w48-1)

Plasmid

#17398

Purpose

Gateway entry 1A vector, no ccDB gene

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

79 Articles

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pENTR1A no ccDB (w48-1)

Plasmid

#17398

Purpose

Gateway entry 1A vector, no ccDB gene

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

79 Articles

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pEBFP2-Nuc

Plasmid

#14893

Purpose

Mammalian expression of EBFP2 blue fluorescent protein with NLS

Depositor

Robert Campbell

Publication

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pEBFP2-Nuc

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pEBFP2-Nuc

Plasmid

#14893

Purpose

Mammalian expression of EBFP2 blue fluorescent protein with NLS

Depositor

Robert Campbell

Publication

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pEBFP2-Nuc

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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NFAT luciferase reporter

Plasmid

#10959

Depositor

Toren Finkel

Publication

Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IL-2 promoter (IL2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

Enlarge
NFAT luciferase reporter

Plasmid

#10959

Depositor

Toren Finkel

Publication

Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IL-2 promoter (IL2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

Enlarge
LSL-Cas9-Rosa26TV

Plasmid

#61408

Purpose

Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

Depositor

Feng Zhang

Publication

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas9
EGFP

Use

CRISPR, Cre/Lox, and Mouse Targeting

Tags

3xFLAG and P2A

Expression

Mammalian

Mutation

human codon optimized

Promoter

CAG

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

Enlarge
LSL-Cas9-Rosa26TV

Plasmid

#61408

Purpose

Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

Depositor

Feng Zhang

Publication

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas9
EGFP

Use

CRISPR, Cre/Lox, and Mouse Targeting

Tags

3xFLAG and P2A

Expression

Mammalian

Mutation

human codon optimized

Promoter

CAG

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

Enlarge
FH-TET1-pEF

Plasmid

#49792

Purpose

This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

Depositor

Anjana Rao

Publication

Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

Insert

Tet methylcytosine dioxygenase 1 (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF-1alpha

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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FH-TET1-pEF

Plasmid

#49792

Purpose

This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

Depositor

Anjana Rao

Publication

Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

Insert

Tet methylcytosine dioxygenase 1 (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF-1alpha

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

Enlarge