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176,087 results

Showing: 1141 - 1160 of 176087 results

  1. Human sgRNA library Brunello in lentiCRISPRv2 (Lentiviral Prep)

    Viral Prep
    #73179-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.
    Depositor
    John Doench, David Root
    Article
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Available Since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only

  3. pLKO.1 puro

    Plasmid
    #8453
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available Since
    July 18, 2005
    Availability
    Academic Institutions and Nonprofits only
    8453-plasmid-map-sequence-id-237918

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  5. pAAV-hSyn-DIO-mCherry (AAV9)

    Viral Prep
    #50459-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available Since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV2/2

    Plasmid
    #232199
    Purpose
    AAV packaging plasmid expressing AAV2 Rep and capsid proteins
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Rep2/Cap2
    Use
    AAV
    Expression
    Mammalian
    Available Since
    Sept. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    232199-plasmid-map-sequence-id-457007

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  7. pAAV-hSyn-hM3D(Gq)-mCherry

    Plasmid
    #50474
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM3D(Gq)-mCherry (CHRM3 Human)
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50474-plasmid-map-sequence-id-471606

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  10. myc-BioID2-MCS

    Plasmid
    #74223
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    Myc and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    74223-plasmid-map-sequence-id-334595

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  11. Flag-miniTurbo

    Plasmid
    #124647
    Purpose
    Expresses Flag-miniTurbo fusion for proximity labeling
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Type
    Empty backbone
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    124647-plasmid-map-sequence-id-242444

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Showing: 1141 - 1160 of 176087 results