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pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

Plasmid

#83842

Purpose

Fluorescent probe for S/G2 transition and G2/M transition, one of two plasmids for the FUCCI4 system

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV5)

Viral Prep

#162378-AAV5

Purpose

Ready-to-use AAV5 particles produced from pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (#162378). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8m-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Promoter

Syn

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only
pcDNA3 flag p53

Plasmid

#10838

Depositor

Thomas Roberts

Article

Gjoerup et al J Virol. 2001 Oct . 75(19):9142-55.

Insert

p53 (TP53 Human)

Tags

flag

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3 flag p53

Plasmid

#10838

Depositor

Thomas Roberts

Article

Gjoerup et al J Virol. 2001 Oct . 75(19):9142-55.

Insert

p53 (TP53 Human)

Tags

flag

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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GFP-Ub

Plasmid

#11928

Purpose

Used for in vivo monitoring of ubiquitin localization.

Depositor

Nico Dantuma

Article

Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

Insert

Ubiquitin (UBC Human)

Tags

GFP

Expression

Mammalian

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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GFP-Ub

Plasmid

#11928

Purpose

Used for in vivo monitoring of ubiquitin localization.

Depositor

Nico Dantuma

Article

Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

Insert

Ubiquitin (UBC Human)

Tags

GFP

Expression

Mammalian

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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Tol2_ISCro4_Deletion_Reporter

Plasmid

#251498

Purpose

Reporter plasmid for monitoring deletion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL components

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 deletion reporter

Expression

Mammalian

Mutation

NA

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCAS

Plasmid

#60847

Purpose

Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

Depositor

Jamie Cate

Article

Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

Inserts

S. pyogenese Cas9
RNA pol III promoter (tRNA-Tyr)
hepatitis delta virus ribozyme, genomic
sgRNA

Use

CRISPR and Synthetic Biology

Tags

NLS/His8 and TTT 3' extension prior to sgRNA

Expression

Bacterial and Yeast

Mutation

L4 is UUCG tetraloop and guide targets LYP1 (CATA…

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCAS

Plasmid

#60847

Purpose

Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

Depositor

Jamie Cate

Article

Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

Inserts

S. pyogenese Cas9
RNA pol III promoter (tRNA-Tyr)
hepatitis delta virus ribozyme, genomic
sgRNA

Use

CRISPR and Synthetic Biology

Tags

NLS/His8 and TTT 3' extension prior to sgRNA

Expression

Bacterial and Yeast

Mutation

L4 is UUCG tetraloop and guide targets LYP1 (CATA…

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre

Plasmid

#13775

Purpose

Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Cre

Use

Cre/Lox

Tags

Myc

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre

Plasmid

#13775

Purpose

Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Cre

Use

Cre/Lox

Tags

Myc

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Article

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

MHCK7

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Article

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

MHCK7

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-miniCMV-TdTomato

Plasmid

#192656

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

TdTomato

Expression

Mammalian

Promoter

U6, miniCMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-miniCMV-TdTomato

Plasmid

#192656

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

TdTomato

Expression

Mammalian

Promoter

U6, miniCMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Human SAM library in lentiSAMv2 backbone

Pooled Library

#1000000078

Purpose

Genome-wide CRISPR gRNA pooled library for activation of human genes

Depositor

Feng Zhang

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pDL278_P23-DsRed-Express2

Plasmid

#121505

Purpose

E. coli-streptococcal shuttle vector that expresses rfp

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

DsRed-Express2

Use

E. coli streptococcal shuttle vector

Promoter

P23

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDL278_P23-DsRed-Express2

Plasmid

#121505

Purpose

E. coli-streptococcal shuttle vector that expresses rfp

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

DsRed-Express2

Use

E. coli streptococcal shuttle vector

Promoter

P23

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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Nluc CD63

Plasmid

#242528

Purpose

This plasmid can be used to quantify CD63 by luminescence signal upon providing substrate, in particular in the secretome, especially in the extracellular vesicles of cells expressing this construct.

Depositor

Clotilde Thery

Article

Grisard et al J Extracell Vesicles. 2022 Jul;11(7):e12

Insert

CD63 (CD63 Human)

Use

Luciferase

Tags

Nanoluciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Plasmid

#83841

Purpose

Fluorescent probe for G1/S transition and M/G1 transition, one of two plasmids for the FUCCI4 cell cycle reporter

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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