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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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243,332 results

Showing: 1141 - 1160 of 243332 results

  13. pSpCas9 (PX165)

    Plasmid
    #48137
    Purpose
    Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)
    Depositor
    Feng Zhang
    Publication
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3XFLAG
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48137-plasmid-map-sequence-id-130642

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  14. pSpCas9 (PX165)

    Plasmid
    #48137
    Purpose
    Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)
    Depositor
    Feng Zhang
    Publication
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3XFLAG
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48137-plasmid-map-sequence-id-130642

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  15. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV Retrograde)

    Viral prep
    #20298-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Oct. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  16. pCRISPomyces-2

    Plasmid
    #61737
    Purpose
    Streptomyces expression of codon-optimized Cas9 and custom gRNA
    Depositor
    Huimin Zhao
    Publication
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    gRNA cassette
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL) and rpsL(XC)-BbsI
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61737-plasmid-map-sequence-id-190162

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  17. pCRISPomyces-2

    Plasmid
    #61737
    Purpose
    Streptomyces expression of codon-optimized Cas9 and custom gRNA
    Depositor
    Huimin Zhao
    Publication
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    gRNA cassette
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL) and rpsL(XC)-BbsI
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61737-plasmid-map-sequence-id-190162

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  20. pMKO.1 GFP

    Plasmid
    #10676
    Purpose
    Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.
    Depositor
    William Hahn
    Type
    Empty backbone
    Use
    RNAi and Retroviral
    Expression
    Mammalian
    Available since
    Dec. 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10676-plasmid-map-sequence-id-139182

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Showing: 1141 - 1160 of 243332 results