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181,067 results

Showing: 1141 - 1160 of 181067 results

  3. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (AAV5)

    Viral Prep
    #100833-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (#100833). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only

  6. ECFP HO

    Plasmid
    #207413
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207413-plasmid-map-sequence-id-421043

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  7. ECFP CD

    Plasmid
    #207414
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207414-plasmid-map-sequence-id-421036

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  8. pOpen-MMLV_RT (mut H)

    Plasmid
    #165546
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
    Use
    Synthetic Biology
    Available Since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165546-plasmid-map-sequence-id-339423

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  10. pAAV-hSyn-EGFP (AAV5)

    Viral Prep
    #50465-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    Dec. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. pAAV-nEF-Coff/Fon-ChR2-mCherry (AAV8)

    Viral Prep
    #137144-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2-mCherry (#137144). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2-mCherry plasmid DNA. nEF-driven, Flp-dependent expression of ChR2-mCherry for optogenetic activation (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    mCherry (Flp-dependent)
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only

  15. pCasSA

    Plasmid
    #98211
    Purpose
    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9
    Depositor
    Quanjiang Ji
    Article
    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    98211-plasmid-map-sequence-id-485072

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  17. pCW57.1

    Plasmid
    #41393
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro
    Depositor
    David Root
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector, doxycycli…
    Expression
    Mammalian
    Promoter
    TRE promoter, Tet ON
    Available Since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41393-plasmid-map-sequence-id-364440

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  18. pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64

    Plasmid
    #203348
    Purpose
    Template for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Christopher Grigsby, Molly Stevens
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Promoter
    T7
    Available Since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203348-plasmid-map-sequence-id-399143

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  19. pSBbi-RB

    Plasmid
    #60522
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60522-plasmid-map-sequence-id-97524

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Showing: 1141 - 1160 of 181067 results