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GST-PRDM16 681-880

Plasmid

#53349

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 681-880

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 455-680

Plasmid

#53348

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 455-680

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
HIP2

Plasmid

#31436

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Huntingtin Interacting protein 2 (UBE2K Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221-SLC29A3_STOP

Plasmid

#161293

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC29A3 (SLC29A3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-MFSD1_STOP

Plasmid

#161082

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD1 (MFSD1 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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ATP1A1_G4_Q118R_EBFP_to_EGFP_Dual_pegRNA

Plasmid

#178100

Purpose

Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and EBFP_To_EGFP pegRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G4 Q118R pegRNA + EBFP to EGFP pegRNA

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pWPXL-p150

Plasmid

#160992

Purpose

Express fulllength of mouse p150 protein

Depositor

Zhiguo Zhang

Article

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Insert

CHAF1A (Chaf1a Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2pA2-drl:APEX2-mCherry

Plasmid

#188945

Purpose

Plasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoter

Depositor

Owen Tamplin

Article

Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

Insert

drl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA

Use

Tol2 destination vector (attr4-r3)

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Pb-TRE3G-TYR-HygR

Plasmid

#195508

Purpose

inducible Piggybac vector expressing human TYR CDS with hygromycin selection

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

TYR (TYR Human)

Use

Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMQ430

Plasmid

#42216

Depositor

Martin Marinus

Article

Calmann et al J Bacteriol. 2003 Aug;185(16):5012-4.

Insert

dam (dam E. coli K-12)

Expression

Bacterial

Promoter

pBAD

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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pMB30

Plasmid

#122247

Purpose

Lentivirus delivery for stable expression of Lb crRNA, has LbDR; Cloning vector for expression of LbCas12a crRNA. It contains BsmBI site for cloning.

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-KrasG13D/sgKras/Cre

Plasmid

#99857

Purpose

Expresses Cre-recombinase, a barcoded HDR template to introduce a G13D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

Depositor

Monte Winslow

Article

Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

Insert

Kras (Kras Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_B2519

Plasmid

#91076

Purpose

Module B, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

OsU3

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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VfAU1-LOV_136

Plasmid

#63632

Purpose

VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by a restriction site

Depositor

Harald Janovjak

Insert

VfAU1-LOV

Expression

Mammalian

Available Since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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MTK0_002

Plasmid

#123960

Purpose

Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB Destination - 2xHS4 - HygroR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-BHLHE40(S243A/E248A)

Plasmid

#110155

Purpose

Expression of human BHLHE40 (S243A/E248A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

Insert

BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)

Tags

HA

Expression

Mammalian

Mutation

S243A/E248A

Promoter

CMV

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIN-turboRFP

Plasmid

#138275

Purpose

Retroviral expression of turboRFP

Depositor

Michael Grusch

Insert

TurboRFP

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a - NDH

Plasmid

#89626

Purpose

Expresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

NAD(P)-dependent oxidoreductase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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Tol2_ISCro4_Inversion_Reporter

Plasmid

#251499

Purpose

Reporter plasmid for monitoring inversion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL components

Depositor

Martin Jinek

Article

Pelea et al Science, 2026. eadz1884, doi:10.1126/sci

Insert

ISCro4 inversion reporter

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2_ISCro4_Insertion_Reporter

Plasmid

#251500

Purpose

Reporter plasmid for monitoring insertion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL and pDonor_ISCro4_(WT)_Donor_ECFP plasmid

Depositor

Martin Jinek

Article

Pelea et al Science, 2026. eadz1884, doi:10.1126/sci

Insert

ISCro4 insertion reporter

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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