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CMV-pDisplay-Halo-T2A-EGFP

Plasmid

#223498

Purpose

Co-expression of HaloTags to outer surface in mammalian cells coupled with a EGFP transfection marker

Depositor

James Frank

Insert

HaloTag and EGFP

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-pDisplay-Halo-T2A-EGFP

Plasmid

#223498

Purpose

Co-expression of HaloTags to outer surface in mammalian cells coupled with a EGFP transfection marker

Depositor

James Frank

Insert

HaloTag and EGFP

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3EF5a-PIK3CA H1047R

Plasmid

#180034

Purpose

Entry vector of PIK3CA H1047R for gateway cloning.

Depositor

Xin Chen

Article

Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Gateway Cloning

Tags

HA tag

Expression

Mammalian

Mutation

H1047R

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pT3EF5a-PIK3CA H1047R

Plasmid

#180034

Purpose

Entry vector of PIK3CA H1047R for gateway cloning.

Depositor

Xin Chen

Article

Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Gateway Cloning

Tags

HA tag

Expression

Mammalian

Mutation

H1047R

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-BG505.SOSIP-gp160

Plasmid

#123273

Purpose

Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations)

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BG505) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; SOSIP mutations

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-BG505.SOSIP-gp160

Plasmid

#123273

Purpose

Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations)

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BG505) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; SOSIP mutations

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GABRA1(A322D)-IRES2-EGFP

Plasmid

#170821

Purpose

human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

Depositor

Tingwei Mu

Article

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Insert

Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

change Alanine 322 to Aspartate

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-GABRA1(A322D)-IRES2-EGFP

Plasmid

#170821

Purpose

human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

Depositor

Tingwei Mu

Article

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Insert

Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

change Alanine 322 to Aspartate

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_G3BP1_G1

Plasmid

#127114

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA G3BP1 (G3BP1 Human)

Use

CRISPR

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_G3BP1_G1

Plasmid

#127114

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA G3BP1 (G3BP1 Human)

Use

CRISPR

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-C7orf59

Plasmid

#42336

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

C7orf59 (LAMTOR4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-HA-C7orf59

Plasmid

#42336

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

C7orf59 (LAMTOR4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pLV_iRFP_P2AT2A_mScarlet-I_Myl9

Plasmid

#172474

Purpose

Mammalian expression of myosin light chain (Myl9) tagged with mScarlet-I and cytoplasmic iRFP as a reference marker.

Depositor

Sean R Collins

Article

Hadjitheodorou et al Nat Commun. 2021 Nov 16;12(1):6619. doi:

Inserts

iRFP670
mScarlet-I-Myl9 (MYL9 Human)

Use

Lentiviral

Tags

mScarlet-I

Expression

Mammalian

Promoter

EF1alpha (with UCOE) and none (same transcript as…

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLV_iRFP_P2AT2A_mScarlet-I_Myl9

Plasmid

#172474

Purpose

Mammalian expression of myosin light chain (Myl9) tagged with mScarlet-I and cytoplasmic iRFP as a reference marker.

Depositor

Sean R Collins

Article

Hadjitheodorou et al Nat Commun. 2021 Nov 16;12(1):6619. doi:

Inserts

iRFP670
mScarlet-I-Myl9 (MYL9 Human)

Use

Lentiviral

Tags

mScarlet-I

Expression

Mammalian

Promoter

EF1alpha (with UCOE) and none (same transcript as…

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-HBXIP

Plasmid

#42328

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

HBXIP (LAMTOR5 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-HA-HBXIP

Plasmid

#42328

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

HBXIP (LAMTOR5 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pTlpA_mCherry_Txe/Axe

Plasmid

#229147

Purpose

Toxin-Antitoxin (Txe/Axe)

Depositor

Shrikrishnan Sankaran

Article

Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

Insert

mCherry, Txe/Axe

Expression

Bacterial

Promoter

PtlpA (mCherry), Txe/Axe (Native)

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pTlpA_mCherry_Txe/Axe

Plasmid

#229147

Purpose

Toxin-Antitoxin (Txe/Axe)

Depositor

Shrikrishnan Sankaran

Article

Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

Insert

mCherry, Txe/Axe

Expression

Bacterial

Promoter

PtlpA (mCherry), Txe/Axe (Native)

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-intron-BaLgp160

Plasmid

#100918

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env with upstream intron

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-intron-BaLgp160

Plasmid

#100918

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env with upstream intron

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge