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PB-TO-ASCL1-DLX2

Plasmid

#182307

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Article

van Voorst et al bioRxiv [Preprint]. 2025 Jul 7:2025.06.3

Inserts

Ascl1 (Ascl1 Mouse)
Dlx2 (Dlx2 Mouse)
puroR-T2A-mycNLS-mTagBFP2

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and pTRE3G-bidirectional

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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LAMP1-mGFP

Plasmid

#34831

Depositor

Esteban Dell'Angelica

Article

Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-

Insert

LAMP1 (LAMP1 Human)

Tags

GSTGSTGSTGA linker, Kozak, and mGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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LAMP1-mGFP

Plasmid

#34831

Depositor

Esteban Dell'Angelica

Article

Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-

Insert

LAMP1 (LAMP1 Human)

Tags

GSTGSTGSTGA linker, Kozak, and mGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pX330-U6-Chimeric_BB-CBh-hSpCas9

Plasmid

#42230

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Has Service

Cloning Grade DNA

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pX330-U6-Chimeric_BB-CBh-hSpCas9

Plasmid

#42230

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Has Service

Cloning Grade DNA

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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mTurquoise2

Plasmid

#198196

Purpose

Spectral correction: Expression of mTurquoise2

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mTurquoise2

Expression

Mammalian

Mutation

A206K

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-M-puro

Plasmid

#125839

Purpose

modifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRI

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Type

Empty backbone

Use

Lentiviral

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SEP-Glur2 (MCA10)

Plasmid

#64941

Purpose

mammalian expression of rat GluR2

Depositor

Jeremy Henley

Article

Ashby et al J Neurosci. 2004 Jun 2;24(22):5172-6.

Insert

GluR2 (Gria2 Rat)

Tags

SEP (superecliptic pHluorin)

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SEP-Glur2 (MCA10)

Plasmid

#64941

Purpose

mammalian expression of rat GluR2

Depositor

Jeremy Henley

Article

Ashby et al J Neurosci. 2004 Jun 2;24(22):5172-6.

Insert

GluR2 (Gria2 Rat)

Tags

SEP (superecliptic pHluorin)

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFZ19

Plasmid

#36184

Purpose

Expression of genes under control of an estrogen-inducible 35S promoter

Depositor

Daniel Voytas

Article

Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

Type

Empty backbone

Use

Plant t-dna vector

Expression

Plant

Promoter

XVE_35S (estrogen-inducible)

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pFZ19

Plasmid

#36184

Purpose

Expression of genes under control of an estrogen-inducible 35S promoter

Depositor

Daniel Voytas

Article

Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

Type

Empty backbone

Use

Plant t-dna vector

Expression

Plant

Promoter

XVE_35S (estrogen-inducible)

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G3BP1-WT

Plasmid

#135997

Purpose

WT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJSK659

Plasmid

#205128

Purpose

Expression of GFP-SnxA in mammalian cells

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1_V5_TurboID_FLAG_Nt

Plasmid

#194072

Purpose

3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Type

Empty backbone

Use

Lentiviral

Tags

V5-TurboID-FLAG

Expression

Mammalian

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.eB

Plasmid

#103005

Purpose

non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

Synthetic construct isolate AAV-PHP.eB VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.eB

Plasmid

#103005

Purpose

non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

Synthetic construct isolate AAV-PHP.eB VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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Jacquere (split-vector)

Pooled Library

#247026

Purpose

Human genome-wide CRISPR knockout library for the use of S. pyogenes Cas9. Each gene is targeted by 3 constructs.

Depositor

John Doench, David Root

Article

Drepanos et al bioRxiv 2025.08.26.672375; doi: https://

Has Service

Lentiviral Prep

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only
LSL-Cas9-Rosa26TV

Plasmid

#61408

Purpose

Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

Cas9
EGFP

Use

CRISPR, Cre/Lox, and Mouse Targeting

Tags

3xFLAG and P2A

Expression

Mammalian

Mutation

human codon optimized

Promoter

CAG

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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LSL-Cas9-Rosa26TV

Plasmid

#61408

Purpose

Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

Cas9
EGFP

Use

CRISPR, Cre/Lox, and Mouse Targeting

Tags

3xFLAG and P2A

Expression

Mammalian

Mutation

human codon optimized

Promoter

CAG

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

Plasmid

#183951

Purpose

Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Insert

APOBEC1 (Apobec1 Rat)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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