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Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization.

Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, Rafelski SM, Gunawardane RN
Mol Biol Cell. 2017 Oct 15;28(21):2854-2874. doi: 10.1091/mbc.E17-03-0209. Epub 2017 Aug 16. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
87420AICSDP-1:PXN-EGFPHomology arms and linker-EGFP sequence for C-terminus tagging of human PXN
87421AICSDP-4:TUBA1B-mEGFPHomology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B
87422AICSDP-10:LMNB1-mEGFPHomology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1
87423AICSDP-8:TOMM20-mEGFPHomology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20
87424AICSDP-9:DSP-mEGFPHomology arms and linker-mEGFP sequence for C-terminus tagging of human DSP
87425AICSDP-15:ACTB-mEGFPHomology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB
87426AICSDP-7:SEC61B-mEGFPHomology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B
87427AICSDP-13:FBL-mEGFPHomology arms and linker-mEGFP sequence for C-terminus tagging of human FBL
87428AICSDP-24:MYH10-mEGFPHomology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10
87429AICSDP-23:TJP1-mEGFPHomology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1

Antibodies from Article