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Addgene
Showing: 1101 - 1120 of 147311 results

  1. pJK541

    Plasmid
    #72447
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein (AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Availability
    Academic Institutions and Nonprofits only
    72447-plasmid-map-sequence-id-133869

    Enlarge

  2. pJK547

    Plasmid
    #72449
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Val319 to Phe
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72449-plasmid-map-sequence-id-133874

    Enlarge

  3. pJK550

    Plasmid
    #72450
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted Val2
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72450-plasmid-map-sequence-id-133877

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  4. pJK552

    Plasmid
    #72451
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins); N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Val319 to Phe and inserted Val2
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72451-plasmid-map-sequence-id-133883

    Enlarge

  5. pJK553

    Plasmid
    #72452
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72452-plasmid-map-sequence-id-133889

    Enlarge

  6. pJK554

    Plasmid
    #72453
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72453-plasmid-map-sequence-id-133893

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  7. pJK565

    Plasmid
    #72454
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Ala/Trp165>Phe mutant (AaPurE1fwf-H59A)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72454-plasmid-map-sequence-id-133894

    Enlarge

  8. pJK569

    Plasmid
    #72455
    Purpose
    Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~51 silent substitutions
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72455-plasmid-map-sequence-id-133906

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  9. pJK573

    Plasmid
    #72456
    Purpose
    Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~57 silent substitutions
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72456-plasmid-map-sequence-id-133910

    Enlarge

  10. pJK578

    Plasmid
    #72457
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Ala/Trp165>Phe mutant (AaPurE1fwf-S57A)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72457-plasmid-map-sequence-id-133895

    Enlarge

  11. pJK585

    Plasmid
    #72458
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Cys/Trp165>Phe mutant (AaPurE1fwf-S57C)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72458-plasmid-map-sequence-id-133896

    Enlarge

  12. pJK586

    Plasmid
    #72459
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Thr/Trp165>Phe mutant (AaPurE1fwf-S57T)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72459-plasmid-map-sequence-id-133898

    Enlarge

  13. pJK616

    Plasmid
    #72460
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with an N-terminal His6 tag (H6TdPurE2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72460-plasmid-map-sequence-id-133770

    Enlarge

  14. pJK626

    Plasmid
    #72461
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to cysteine, tyrosine-…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72461-plasmid-map-sequence-id-133899

    Enlarge

  15. pJK627

    Plasmid
    #72462
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp/Tyr154>Phe mutant (AaPurE1-S57D,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to aspartate, tyrosine…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72462-plasmid-map-sequence-id-133901

    Enlarge

  16. pJK628

    Plasmid
    #72463
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Ala/Tyr154>Phe mutant (AaPurE1-S57A,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to alanine, tyrosine-1…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72463-plasmid-map-sequence-id-133902

    Enlarge

  17. pJK629

    Plasmid
    #72464
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Thr/Tyr154>Phe mutant (AaPurE1-S57T,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to threonine, tyrosine…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72464-plasmid-map-sequence-id-133903

    Enlarge

  18. pJK632

    Plasmid
    #72465
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Val/Tyr154>Phe mutant (AaPurE1-S57V,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to valine, tyrosine-15…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72465-plasmid-map-sequence-id-133904

    Enlarge

  19. pJK636

    Plasmid
    #72466
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72466-plasmid-map-sequence-id-133767

    Enlarge

  20. pJK640

    Plasmid
    #72467
    Purpose
    Encodes Escherichia coli evgA
    Depositor
    T. J. Kappock
    Insert
    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72467-plasmid-map-sequence-id-134013

    Enlarge

Showing: 1101 - 1120 of 147311 results