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Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy.

Liu Z, Lu X, Villette V, Gou Y, Colbert KL, Lai S, Guan S, Land MA, Lee J, Assefa T, Zollinger DR, Korympidou MM, Vlasits AL, Pang MM, Su S, Cai C, Froudarakis E, Zhou N, Patel SS, Smith CL, Ayon A, Bizouard P, Bradley J, Franke K, Clandinin TR, Giovannucci A, Tolias AS, Reimer J, Dieudonne S, St-Pierre F
Cell. 2022 Aug 16. pii: S0092-8674(22)00916-3. doi: 10.1016/j.cell.2022.07.013. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
179458pcDNA3.1/Puro-CAG-JEDI-2PGenetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)
179459pAAV-EF1a-DIO-JEDI-2P-Kv-WPREDouble floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)
179460pAAV-EF1a-DIO-JEDI-2P-WPREDouble floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector under the control of the mammalian promoter (EF1a)
179461pJFRC7-JEDI-2PGenetically encoded voltage indicator (GEVI) JEDI-2P under the promoter hsp70 for Drosophila (insect) expression
179462pcDNA3.1/Puro-CAG-JEDI-2P-KvSoma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)
179463pAAV-hSyn-JEDI-2P-Kv-WPRESoma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn
179464pAAV-hSyn-JEDI-2P-WPREGenetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn
179465pAAV-CaMKIIa-JEDI-2P-Kv-WPRESoma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII
179466pAAV-CaMKIIa-JEDI-2P-WPREGenetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII
179469pAAV-GFAP-JEDI-2P-WPREGenetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for astrocytes specific expression using the promoter GFAP

Antibodies from Article