Addgene

We narrowed to 13,610 results for: pET

Showing: 10861 - 10880 of 13610 results

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pRL443

Plasmid

#70261

Purpose

Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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p7XC3H

Plasmid

#47065

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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KLC2

Plasmid

#40735

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

KLC2 (KLC2 Human)

Use

Tags

6xHis TEV

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pHMM

Plasmid

#67717

Purpose

Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein

Depositor

Robert Batey, Jeffrey Kieft

Article

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Hexahistidine/Maltose binding protein/MS2 coat protein fusion

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7 RNA polymerase

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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p3962 pcDNA3-IRF3-FL

Plasmid

#22860

Depositor

Peter Howley

Article

Kirshner et al J Virol. 2005 Jul . 79(14):9320-4.

Insert

IRF3 (IRF3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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p3258 pCMV hUBC9 wt HA

Plasmid

#14438

Depositor

Peter Howley

Article

Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

Insert

UBC9 (UBE2I Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pSRK2706

Plasmid

#78571

Purpose

Expresses untagged human rhinovirus 3C protease in E. coli

Depositor

David Waugh

Article

Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

Insert

human rhinovirus 3C protease (HRVBgp1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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CAPZA1-TwinStrepII

Plasmid

#83193

Purpose

Expression of tagged protein in mammalian cells

Depositor

Peter Barr-Gillespie

Insert

CAPZA1 (Capza1 Mouse)

Use

Tags

Twin Strep Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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HAT1

Plasmid

#25239

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

HAT1_05 (HAT1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2010

Availability

Industry, Academic Institutions, and Nonprofits
MtMscL

Plasmid

#79028

Purpose

Mycobacterium tuberculosis MscL

Depositor

Douglas Rees

Article

Chang et al Science. 1998 Dec 18;282(5397):2220-6.

Insert

MtMscL

Use

Tags

10x Histidine Tag

Expression

Mutation

Promoter

T7 Promoter

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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p3692 HPV-16 E1

Plasmid

#10859

Depositor

Peter Howley

Article

Sakai et al J Virol. 1996 Mar . 70(3):1602-11.

Insert

HPV-16 E1 (E1 Human papillomavirus)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pXPG

Plasmid

#71248

Purpose

improved luciferase reporter gene plasmid

Depositor

Peter Cockerill

Article

Bert et al Plasmid. 2000 Sep;44(2):173-82.

Type

Empty backbone

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

none

Available since

Nov. 12, 2015

Availability

Academic Institutions and Nonprofits only

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p3868 HA-E6-AP

Plasmid

#8648

Depositor

Peter Howley

Article

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Insert

E6-AP (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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p7XNH3

Plasmid

#47064

Purpose

FX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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p4054 HA-E6AP isoform II

Plasmid

#8658

Depositor

Peter Howley

Article

Kao et al J Virol 2000 Jul;74(14):6408-17.

Insert

E6-AP isoform II (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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pSL4529 (pTnpA)

Plasmid

#210869

Purpose

Expresses GstTnpA from pLac promoter

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

GstTnpA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Ecoli cpGFP

Plasmid

#215713

Purpose

Expresses cpGFP in Ecoli

Depositor

Katharine Diehl

Article

Smith et al bioRxiv 2023.12.31.573774

Insert

cpGFP

Use

Tags

6xHis

Expression

Bacterial

Mutation

circularly permuted GFP

Promoter

T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pPTbsr

Plasmid

#117696

Purpose

Blasticidin-S resistance Vector for Phaeodactylum tricornutum

Depositor

Peter Kroth

Article

Buck et al PeerJ. 2018 Nov 14;6:e5884. doi: 10.7717

Type

Empty backbone

Use

Vector for phaeodactylum tricornutum

Tags

Expression

Mutation

Promoter

fcpB

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pNICE-NL2A

Plasmid

#15259

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 2 with insert A (Nlgn2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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6C1A (MBD2)

Plasmid

#119958

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Insert

MBD2 (MBD2 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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