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Addgene

MtMscL
(Plasmid #79028)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79028 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET19b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5724
  • Total vector size (bp) 6180

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MtMscL
  • Alt name
    TbMscL
  • Species
    Mycobacterium tuberculosis
  • Insert Size (bp)
    456
  • Promoter T7 Promoter
  • Tag / Fusion Protein
    • 10x Histidine Tag (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MtMscL was a gift from Douglas Rees (Addgene plasmid # 79028 ; http://n2t.net/addgene:79028 ; RRID:Addgene_79028)
  • For your References section:

    Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel. Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC. Science. 1998 Dec 18;282(5397):2220-6. PubMed 9856938