MtMscL
(Plasmid
#79028)
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PurposeMycobacterium tuberculosis MscL
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 79028 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET19b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5724
- Total vector size (bp) 6180
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMtMscL
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Alt nameTbMscL
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SpeciesMycobacterium tuberculosis
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Insert Size (bp)456
- Promoter T7 Promoter
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Tag
/ Fusion Protein
- 10x Histidine Tag (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 Forward
- 3′ sequencing primer T7 Reverse (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MtMscL was a gift from Douglas Rees (Addgene plasmid # 79028 ; http://n2t.net/addgene:79028 ; RRID:Addgene_79028) -
For your References section:
Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel. Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC. Science. 1998 Dec 18;282(5397):2220-6. PubMed 9856938