Skip to main content
Addgene

p7XNH3
(Plasmid #47064)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47064 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET26b
  • Backbone manufacturer
    Novagen
  • Backbone size (bp) 6976
  • Modifications to backbone
    modified for compatibility with FX cloning and added tags/fusion proteins
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tags / Fusion Proteins
    • decaHis (10x His) (N terminal on backbone)
    • 3C protease site (PreScission site) (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    Unknown

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p7XNH3 was a gift from Raimund Dutzler & Eric Geertsma (Addgene plasmid # 47064 ; http://n2t.net/addgene:47064 ; RRID:Addgene_47064)
  • For your References section:

    A versatile and efficient high-throughput cloning tool for structural biology. Geertsma ER, Dutzler R. Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25. 10.1021/bi200178z PubMed 21410291