pINIT_cat
(Plasmid
#46858)
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Purpose(Empty Backbone) FX sequencing vector for subcloning, chloramphenicol marker
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46858 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
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Backbone manufacturerunknown
- Backbone size (bp) 4980
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Vector typesequencing vector
- Promoter none
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Growth instructionsdue to the presence of the negative selection marker sacB the growth medium should not contain sucrose
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Copy numberLow Copy
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pINIT_cat was a gift from Raimund Dutzler & Eric Geertsma (Addgene plasmid # 46858 ; http://n2t.net/addgene:46858 ; RRID:Addgene_46858) -
For your References section:
A versatile and efficient high-throughput cloning tool for structural biology. Geertsma ER, Dutzler R. Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25. 10.1021/bi200178z PubMed 21410291