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Transposon-encoded nucleases use guide RNAs to promote their selfish spread.

Meers C, Le HC, Pesari SR, Hoffmann FT, Walker MWG, Gezelle J, Tang S, Sternberg SH
Nature. 2023 Sep 27. doi: 10.1038/s41586-023-06597-1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
210869pSL4529 (pTnpA)Expresses GstTnpA from pLac promoter
210870pSL4624 (pEffector_TnpB1,ωRNA)Expresses GstTnpB1 and native ωRNA from constitutive promoters
210871pSL4369 (pEffector_TnpB2,ωRNA)Expresses GstTnpB2 and native ωRNA from constitutive promoters
210872pSL4672 (pEffector_TnpB3,ωRNA)Expresses GstTnpB3 and native ωRNA from constitutive promoters
210873pSL4673 (pEffector_TnpB4,ωRNA)Expresses GstTnpB4 and native ωRNA from constitutive promoters
210874pSL4164 (pEffector_IscB,ωRNA)Expresses GstIscB and native ωRNA from constitutive promoters
210875pSL5200 (pDonor_ISGst3)Harbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458

Antibodies from Article