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Plasmid#79025PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only
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T7m
Plasmid#121030PurposeMoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertS. pyogenes tracrRNA terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pREP41xN_ccdB
Plasmid#79023PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP42xN_ccdB
Plasmid#79024PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas9-sgAAVS1-1
Plasmid#129726PurposeExpressing SpCas9 and sgAAVS1-1DepositorInsertSpCas9 and sgAAVS1-1
UseCRISPRTagsP2A-PuroExpressionMammalianMutationPromoterhU6Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9-Blast
Plasmid#52962PurposeExpresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.DepositorHas ServiceLentiviral PrepInsertsCas9
Blasticidin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS-NSAvailable sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP_NbALFA
Plasmid#136626PurposePlasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusionDepositorInsertEGFP-NbALFA
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG1159 TOM20-ECFP-FIREmate
Plasmid#196693PurposeExpresses TOM20-ECFP-FIREmate in mammalian cellsDepositorInsertTOM20-ECFP-FIREmate
UseTagsFIREmateExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAureus-TnCAM
Plasmid#199807Purposevector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureusDepositorInserttransposon cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG1237 FIREmate-ECFP-Fis1
Plasmid#205173PurposeExpresses FIREmate-ECFP-Fis1 in mammalian cellsDepositorInsertFIREmate-ECFP-Fis1
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-miRFP670nano3
Plasmid#184664PurposeMonomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmidDepositorInsertmiRFP670nano3
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Cherry
Plasmid#170510PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCHARGE3
Plasmid#24658DepositorInsertTurbo
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbALFA
Plasmid#184678PurposeNIR-Fb to ALFA-tagDepositorInsertNIR-FbALFA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
HI.fate.UB
Plasmid#191556PurposeMonitor HIV-1 fateDepositorInsertHIV-1 incomplete genome
UseLentiviralTagsExpressionMutationenv-deleted + vpr mutation(s)PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
HI.fate.SFFV-E
Plasmid#191555PurposeMonitor HIV-1 fateDepositorInsertHIV-1 incomplete genome
UseLentiviralTagsExpressionMutationenv-deleted + vpr mutation(s)PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
HI.fate.E
Plasmid#191553PurposeMonitor HIV-1 fateDepositorInsertHIV-1 incomplete genome
UseLentiviralTagsExpressionMutationenv-deleted + vpr mutation(s)PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
HI.fate.SFFV
Plasmid#191554PurposeMonitor HIV-1 fateDepositorInsertHIV-1 incomplete genome
UseLentiviralTagsExpressionMutationenv-deleted + vpr mutation(s)PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG1186 Lyn11-ECFP-FIREmate
Plasmid#205168PurposeExpresses Lyn11-ECFP-FIREmate in mammalian cellsDepositorInsertLyn11-ECFP-FIREmate
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG1171 FIREmate-ECFP-Giantin
Plasmid#205166PurposeExpresses FIREmate-ECFP-Giantin in mammalian cellsDepositorInsertFIREmate-ECFP-Giantin
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only