Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator.
Shemesh OA, Linghu C, Piatkevich KD, Goodwin D, Celiker OT, Gritton HJ, Romano MF, Gao R, Yu CJ, Tseng HA, Bensussen S, Narayan S, Yang CT, Freifeld L, Siciliano CA, Gupta I, Wang J, Pak N, Yoon YG, Ullmann JFP, Guner-Ataman B, Noamany H, Sheinkopf ZR, Park WM, Asano S, Keating AE, Trimmer JS, Reimer J, Tolias AS, Bear MF, Tye KM, Han X, Ahrens MB, Boyden ES
Neuron. 2020 Jun 26. pii: S0896-6273(20)30398-6. doi: 10.1016/j.neuron.2020.05.029.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
158755 | pAAV-Syn-SomaGCaMP6f1 | Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2. |
158756 | pAAV-Syn-SomaGCaMP6f2 | Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f. |
158757 | pAAV-CAG-SomaGCaMP6f2 | Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f. |
158758 | pAAV-Syn-nullCoChR-GCaMP6f-Kv2.1 | Cell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the optogenetic protein CoChR mutated to have 0 current), followed by GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1). |
158759 | pAAV-Syn-SomaGCaMP7 | Cell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f. |
158760 | elavl3:SomaGCaMP7f | Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f. |
182941 | pAAV-EF1a-FLEX-SomaGCaMP7 | Cre-dependent expression of cell body-targeted GCaMP7f, under EF1a promoter. As bright as conventional GCaMP7f. |
182942 | pAAV-CAG-FLEX-SomaGCaMP7 | Cre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f. |
182943 | pAAV-Syn-Soma-jGCaMP8f | Cell body-targeted jGCaMP8f, under synapsin promoter. |