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Showing: 3681 - 3700 of 157763 results

  12. biGBac Kit

    Plasmid Kit
    #1000000088
    Purpose
    Generation of baculoviral transfer vectors coding up to 25 protein complex subunits in two Gibson assembly steps. Multisubunit protein complexes can be generated in baculovirus-infected insect cells.
    Depositor
    Jan-Michael Peters
    Available since
    Oct. 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. pSAG1::CAS9-U6::sgUPRT

    Plasmid
    #54467
    Purpose
    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.
    Depositor
    David Sibley
    Article
    Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.
    Insert
    SAG1 5' UTR
    Use
    CRISPR; Toxoplasma expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    54467-plasmid-map-sequence-id-84808

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  18. pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP (AAV8)

    Viral Prep
    #137148-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP (#137148). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of Arch3.3-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    INTRSECT 2.0 Project, Karl Deisseroth
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    EYFP (Cre and Flp-dependent)
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only

  20. PEA1-Puro

    Plasmid
    #171991
    Purpose
    Delivers all prime editing nickase components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171991-plasmid-map-sequence-id-338127

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Showing: 3681 - 3700 of 157763 results