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Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells of Behaving Mammals.

Fenno LE, Ramakrishnan C, Kim YS, Evans KE, Lo M, Vesuna S, Inoue M, Cheung KYM, Yuen E, Pichamoorthy N, Hong ASO, Deisseroth K
Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 10.1016/j.neuron.2020.06.003. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
137119pAAV-EF1a-Con/Fon-GCaMP6MIntersectional viral expression of GCaMP6M in cells expressing both Cre AND Flp
137120pAAV-Ef1a-Con/Foff 2.0-GCaMP6MIntersectional viral expression of GCaMP6M in cells expressing Cre AND NOT Flp
137121pAAV-Ef1a-Coff/Fon-GCaMP6MIntersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre
137122pAAV-Ef1a-Con/Fon-GCaMP6FIntersectional viral expression of GCaMP6F in cells expressing both Cre AND Flp
137123pAAV-Ef1a-Con/Foff 2.0-GCaMP6FIntersectional viral expression of GCaMP6F in cells expressing Cre AND NOT Flp
137124pAAV-Ef1a-Coff/Fon-GCaMP6FIntersectional viral expression of GCaMP6F in cells expressing Flp AND NOT Cre
137125pAAV-Ef1a-sRGECOViral expression of sRGECO
137126pAAV-Ef1a-Con/Fon-sRGECOIntersectional viral expression of sRGECO in cells expressing both Cre AND Flp
137127pAAV-Ef1a-Con/Foff 2.0-sRGECOIntersectional viral expression of sRGECO in cells expressing Cre AND NOT Flp
137128pAAV-Ef1a-Coff/Fon-sRGECOIntersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre
137129pAAV-Ef1a-Con/Fon-BFPIntersectional viral expression of BFP in cells expressing both Cre AND Flp
137130pAAV-Ef1a-Con/Foff 2.0-BFPIntersectional viral expression of BFP in cells expressing Cre AND NOT Flp
137131pAAV-Ef1a-Coff/Fon-BFPIntersectional viral expression of BFP in cells expressing Flp AND NOT Cre
137132pAAV-Ef1a-Con/Fon-mCherryIntersectional viral expression of mCherry in cells expressing both Cre AND Flp
137133pAAV-Ef1a-Con/Foff 2.0-mCherryIntersectional viral expression of mCherry in cells expressing Cre AND NOT Flp
137134pAAV-Ef1a-Coff/Fon-mCherryIntersectional viral expression of mCherry in cells expressing Flp AND NOT Cre
137135pAAV-Ef1a-oScarletViral expression of oScarlet
137136pAAV-Ef1a-Con/Fon-oScarletIntersectional viral expression of oScarlet in cells expressing both Cre AND Flp
137137pAAV-Ef1a-Con/Foff 2.0-oScarletIntersectional viral expression of oScarlet in cells expressing Cre AND NOT Flp
137138pAAV-Ef1a-Coff/Fon-oScarletIntersectional viral expression of oScarlet in cells expressing Flp AND NOT Cre
137139pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFPIntersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing both Cre AND Flp
137140pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFPIntersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Cre AND NOT Flp
137141pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFPIntersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Flp AND NOT Cre
137142pAAV-nEF-Con/Fon-ChR2-mCherryIntersectional viral expression of ChR2-mCherry in cells expressing both Cre AND Flp
137143pAAV-nEF-Con/Foff 2.0-ChR2-mCherryIntersectional viral expression of ChR2-mCherry in cells expressing Cre AND NOT Flp
137144pAAV-nEF-Coff/Fon-ChR2-mCherryIntersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT Cre
137145pAAV-nEF-Con/Fon-bREACHes-EYFPIntersectional viral expression of bREACHes-EYFP in cells expressing both Cre AND Flp
137146pAAV-nEF-Con/Foff 2.0-bREACHes-EYFPIntersectional viral expression of bREACHes-EYFP in cells expressing Cre AND NOT Flp
137147pAAV-nEF-Coff/Fon-bREACHes-EYFPIntersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT Cre
137148pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFPIntersectional viral expression of Arch3.3-EYFP in cells expressing both Cre AND Flp
137149pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFPIntersectional viral expression of Arch3.3-EYFP in cells expressing Cre AND NOT Flp
137150pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFPIntersectional viral expression of Arch3.3-EYFP in cells expressing Flp AND NOT Cre
137151pAAV-nEF-NpHR3.3-EYFPViral expression of NpHR3.3-EYFP
137152pAAV-nEF-Con/Fon-NpHR3.3-EYFPIntersectional viral expression of NpHR3.3-EYFP in cells expressing both Cre AND Flp
137153pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFPIntersectional viral expression of NpHR3.3-EYFP in cells expressing Cre AND NOT Flp
137154pAAV-nEF-Coff/Fon-NpHR3.3-EYFPIntersectional viral expression of NpHR3.3-EYFP in cells expressing Flp AND NOT Cre
137155pAAV-nEF-Con/Fon-iC++-EYFPIntersectional viral expression of iC++-EYFP in cells expressing both Cre AND Flp
137156pAAV-nEF-Con/Foff 2.0-iC++-EYFPIntersectional viral expression of ic++-EYFP in cells expressing Cre AND NOT Flp
137157pAAV-nEF-Coff/Fon-iC++-EYFPIntersectional viral expression of iC++-EYFP in cells expressing Flp AND NOT Cre
137158pAAV-nEF-ChRmine-mScarletViral expression of ChRmine-p2a-mScarlet
137159pAAV-nEF-Con/Fon-ChRmine-oScarletIntersectional viral expression of ChRmine-p2a-oScarlet in cells expressing both Cre AND Flp
137160pAAV-nEF-Coff/Fon-ChRmine-oScarletIntersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Flp AND NOT Cre
137161pAAV-nEF-Con/Foff 2.0-ChRmine-oScarletIntersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Cre AND NOT Flp
137162pAAV-Ef1a-Con/Foff 2.0-EYFPIntersectional viral expression of EYFP in cells expressing Cre AND NOT Flp
137163pAAV-nEF-Con/Foff 2.0-ChR2-EYFPIntersectional viral expression of ChR2-EYFP in cells expressing Cre AND NOT Flp
137164pAAV-nEF-Con/Fon/Von eYFPIntersectional viral expression of EYFP in cells expressing Cre AND Flp AND Vcre
137165pAAV-Ef1a-Con/Fon/Von GCaMP 6mIntersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND Vcre

Antibodies from Article