Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells of Behaving Mammals.
Fenno LE, Ramakrishnan C, Kim YS, Evans KE, Lo M, Vesuna S, Inoue M, Cheung KYM, Yuen E, Pichamoorthy N, Hong ASO, Deisseroth K
Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 10.1016/j.neuron.2020.06.003.
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
137119 | pAAV-EF1a-Con/Fon-GCaMP6M | Intersectional viral expression of GCaMP6M in cells expressing both Cre AND Flp |
137120 | pAAV-Ef1a-Con/Foff 2.0-GCaMP6M | Intersectional viral expression of GCaMP6M in cells expressing Cre AND NOT Flp |
137121 | pAAV-Ef1a-Coff/Fon-GCaMP6M | Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre |
137122 | pAAV-Ef1a-Con/Fon-GCaMP6F | Intersectional viral expression of GCaMP6F in cells expressing both Cre AND Flp |
137123 | pAAV-Ef1a-Con/Foff 2.0-GCaMP6F | Intersectional viral expression of GCaMP6F in cells expressing Cre AND NOT Flp |
137124 | pAAV-Ef1a-Coff/Fon-GCaMP6F | Intersectional viral expression of GCaMP6F in cells expressing Flp AND NOT Cre |
137125 | pAAV-Ef1a-sRGECO | Viral expression of sRGECO |
137126 | pAAV-Ef1a-Con/Fon-sRGECO | Intersectional viral expression of sRGECO in cells expressing both Cre AND Flp |
137127 | pAAV-Ef1a-Con/Foff 2.0-sRGECO | Intersectional viral expression of sRGECO in cells expressing Cre AND NOT Flp |
137128 | pAAV-Ef1a-Coff/Fon-sRGECO | Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre |
137129 | pAAV-Ef1a-Con/Fon-BFP | Intersectional viral expression of BFP in cells expressing both Cre AND Flp |
137130 | pAAV-Ef1a-Con/Foff 2.0-BFP | Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp |
137131 | pAAV-Ef1a-Coff/Fon-BFP | Intersectional viral expression of BFP in cells expressing Flp AND NOT Cre |
137132 | pAAV-Ef1a-Con/Fon-mCherry | Intersectional viral expression of mCherry in cells expressing both Cre AND Flp |
137133 | pAAV-Ef1a-Con/Foff 2.0-mCherry | Intersectional viral expression of mCherry in cells expressing Cre AND NOT Flp |
137134 | pAAV-Ef1a-Coff/Fon-mCherry | Intersectional viral expression of mCherry in cells expressing Flp AND NOT Cre |
137135 | pAAV-Ef1a-oScarlet | Viral expression of oScarlet |
137136 | pAAV-Ef1a-Con/Fon-oScarlet | Intersectional viral expression of oScarlet in cells expressing both Cre AND Flp |
137137 | pAAV-Ef1a-Con/Foff 2.0-oScarlet | Intersectional viral expression of oScarlet in cells expressing Cre AND NOT Flp |
137138 | pAAV-Ef1a-Coff/Fon-oScarlet | Intersectional viral expression of oScarlet in cells expressing Flp AND NOT Cre |
137139 | pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP | Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing both Cre AND Flp |
137140 | pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP | Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Cre AND NOT Flp |
137141 | pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP | Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Flp AND NOT Cre |
137142 | pAAV-nEF-Con/Fon-ChR2-mCherry | Intersectional viral expression of ChR2-mCherry in cells expressing both Cre AND Flp |
137143 | pAAV-nEF-Con/Foff 2.0-ChR2-mCherry | Intersectional viral expression of ChR2-mCherry in cells expressing Cre AND NOT Flp |
137144 | pAAV-nEF-Coff/Fon-ChR2-mCherry | Intersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT Cre |
137145 | pAAV-nEF-Con/Fon-bREACHes-EYFP | Intersectional viral expression of bREACHes-EYFP in cells expressing both Cre AND Flp |
137146 | pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP | Intersectional viral expression of bREACHes-EYFP in cells expressing Cre AND NOT Flp |
137147 | pAAV-nEF-Coff/Fon-bREACHes-EYFP | Intersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT Cre |
137148 | pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP | Intersectional viral expression of Arch3.3-EYFP in cells expressing both Cre AND Flp |
137149 | pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP | Intersectional viral expression of Arch3.3-EYFP in cells expressing Cre AND NOT Flp |
137150 | pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP | Intersectional viral expression of Arch3.3-EYFP in cells expressing Flp AND NOT Cre |
137151 | pAAV-nEF-NpHR3.3-EYFP | Viral expression of NpHR3.3-EYFP |
137152 | pAAV-nEF-Con/Fon-NpHR3.3-EYFP | Intersectional viral expression of NpHR3.3-EYFP in cells expressing both Cre AND Flp |
137153 | pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFP | Intersectional viral expression of NpHR3.3-EYFP in cells expressing Cre AND NOT Flp |
137154 | pAAV-nEF-Coff/Fon-NpHR3.3-EYFP | Intersectional viral expression of NpHR3.3-EYFP in cells expressing Flp AND NOT Cre |
137155 | pAAV-nEF-Con/Fon-iC++-EYFP | Intersectional viral expression of iC++-EYFP in cells expressing both Cre AND Flp |
137156 | pAAV-nEF-Con/Foff 2.0-iC++-EYFP | Intersectional viral expression of ic++-EYFP in cells expressing Cre AND NOT Flp |
137157 | pAAV-nEF-Coff/Fon-iC++-EYFP | Intersectional viral expression of iC++-EYFP in cells expressing Flp AND NOT Cre |
137158 | pAAV-nEF-ChRmine-mScarlet | Viral expression of ChRmine-p2a-mScarlet |
137159 | pAAV-nEF-Con/Fon-ChRmine-oScarlet | Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing both Cre AND Flp |
137160 | pAAV-nEF-Coff/Fon-ChRmine-oScarlet | Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Flp AND NOT Cre |
137161 | pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet | Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Cre AND NOT Flp |
137162 | pAAV-Ef1a-Con/Foff 2.0-EYFP | Intersectional viral expression of EYFP in cells expressing Cre AND NOT Flp |
137163 | pAAV-nEF-Con/Foff 2.0-ChR2-EYFP | Intersectional viral expression of ChR2-EYFP in cells expressing Cre AND NOT Flp |
137164 | pAAV-nEF-Con/Fon/Von eYFP | Intersectional viral expression of EYFP in cells expressing Cre AND Flp AND Vcre |
137165 | pAAV-Ef1a-Con/Fon/Von GCaMP 6m | Intersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND Vcre |