Addgene

202,558 results

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FLAG.PKCzeta

Plasmid

#10799

Depositor

Alex Toker

Article

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Insert

PKC zeta (Prkcz Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
FLAG.PKCzeta

Plasmid

#10799

Depositor

Alex Toker

Article

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Insert

PKC zeta (Prkcz Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
nAChR alpha4 WT

Plasmid

#24271

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

nAChR alpha4 WT (Chrna4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only
nAChR alpha4 WT

Plasmid

#24271

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

nAChR alpha4 WT (Chrna4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn Con/Fon EYFP (AAV8)

Viral Prep

#55650-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-hSyn Con/Fon EYFP (#55650). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon EYFP plasmid DNA. Synapsin driven, Cre and Flp-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Promoter

Syn

Tags

EYFP (Cre- and Flp-dependent)

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only
pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

Plasmid

#122084

Purpose

Express M.EcoGII fused to TRF1 with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-TERF1

Use

Retroviral

Tags

V5 tag on Nter of TERF1

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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20XUAS IVS CsChrimson tdtomato_tr

Plasmid

#111544

Purpose

Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chrimson_tdtomato_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET28 Set9 wt

Plasmid

#24082

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Use

Tags

FLAG and His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pET28 Set9 wt

Plasmid

#24082

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Use

Tags

FLAG and His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
AAV-Syn-PSD95.FingR-eGFP-CCR5TC

Plasmid

#125693

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pQTEV-CIP29

Plasmid

#34822

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

cytokine induced protein 29 kDa (SARNP Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pQTEV-CIP29

Plasmid

#34822

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

cytokine induced protein 29 kDa (SARNP Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pYLsgRNA-AtU3b/LacZ

Plasmid

#66199

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

AtU3b

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYLsgRNA-AtU3b/LacZ

Plasmid

#66199

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

AtU3b

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-AMPH1-BAR-mCherry

Plasmid

#85130

Purpose

BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry (AMPH Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pGR475

Plasmid

#213784

Purpose

Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker

Depositor

Chang Liu

Insert

WT TP-DNAP1 (recoded)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-FLAG-HIS3MX6

Plasmid

#20750

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

Expression

Mutation

Promoter

Available since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-FLAG-HIS3MX6

Plasmid

#20750

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

Expression

Mutation

Promoter

Available since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+MCS-P2A-sfGFP

Plasmid

#74668

Purpose

Plasmid for expression of wild-type or mutant cDNA fragments (mutation reporter) or complete cDNAs in frame with superfolderGFP.

Depositor

Jason Berman

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+MCS-P2A-sfGFP

Plasmid

#74668

Purpose

Plasmid for expression of wild-type or mutant cDNA fragments (mutation reporter) or complete cDNAs in frame with superfolderGFP.

Depositor

Jason Berman

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge