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Plasmid#188471PurposeLentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)DepositorInsertenCas12a-HF (gift from Dr. Keith Joung)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1180 U6-reci Gag-pol v2
Plasmid#201915PurposeCas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-pol
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSFV3
Plasmid#92072PurposeEmpty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.DepositorTypeEmpty backboneUseEmpty expression plasmid, for run-off transcripti…TagsExpressionMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pUC57-LbNOX
Plasmid#75285PurposeThis plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertLbNOX
UseTagsC-terminal FLAG tag with linkerExpressionMutationHuman codon optimizedPromoterAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6b
Plasmid#215820PurposeExpress CBE6b (with SpCas9) in mammalian cellsDepositorInsertCBE6b-SpCas9-2xUGI (cas9 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST (AAV9)
Viral Prep#105448-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST (#105448). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST plasmid DNA. hSyn-driven expression of ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmRuby2Available sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(4,5)P2 Probe
Plasmid#211513PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteriaDepositorInsertPLCdelta1-PH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/DEST/hTfR-HA
Plasmid#69610Purposehuman transferrin receptor with an HA tag fused at the C-terminusDepositorInserthuman transferrin receptor (TFRC Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_AGIA-AirID
Plasmid#182210PurposeExpress AirID in mammalian cells.DepositorInsertAGIA-AirID
UseTagsAGIA-tagExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tdTomato (AAV8)
Viral Prep#28306-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Cre-dependent)Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT375
Plasmid#125616PurposeExpresses evoAPOBEC1-BE4max-NG in mammalian cellsDepositorInsertevoAPOBEC1-BE4max-NG
UseTagsExpressionMammalianMutationE4K H109N H122L D124N R154H A165S P201S F205SPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_23C
Plasmid#91140PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:barDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA
Plasmid#84474PurposepAAV-RAM-NLS-mKate2DepositorInsertmKate2
UseAAVTagsNuclear localization signal of SV40 large T antig…ExpressionMutationPromoterTREAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro mCherry-EGFP-LC3B
Plasmid#22418Purposeretroviral expression of mCherry, EGFP-tagged LC3DepositorInsertmicrotubule-associated protein 1 light chain 3 beta (MAP1LC3B Human)
UseRetroviralTagseGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
SFFV-DTAG-NTERM-GFP
Plasmid#185760PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Grx1-roCherry
Plasmid#141068PurposeFluorescent biosensor for 2GSH/GSSG ratioDepositorInsertGrx1-roCherry
UseTagsExpressionMammalianMutationChanged Alanine 150 to Cysteine, inserted Threon…PromoterCMV, SP6Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22C
Plasmid#91135PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-WT human MyoD-T2A-dsRedExpress2
Plasmid#60628PurposeExpresses WT human MyoD and dsRedExpress2 in response to doxycyclineDepositorInsertVP64 mouse MyoD (MYOD1 Human)
UseLentiviralTagsExpressionMutationPromoterTREAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Dual-sgRNA_hU6-mU6
Plasmid#154194PurposeLentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only