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pAAV-CAG-FLEx-iSeroSnFR-EnhancedExport

Plasmid

#129180

Purpose

Fluorescent reporter for serotonin imaging (conditional + membrane localization tag with enhanced export sequence)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Use

AAV

Tags

Ig-kappa leader, Myc, and PDGFR + enhanced ER exp…

Promoter

CAG

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-ChR2-mCherry-Fishell-3

Plasmid

#83898

Purpose

ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

ChR2

Use

AAV

Expression

Mammalian

Promoter

mDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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013LP-pAAV-ANF-657-EmGFP-pA

Plasmid

#120267

Purpose

AAV vector for Atrial specific EmGFP expression

Depositor

Xander Wehrens

Article

Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

Insert

EmGFP

Use

AAV

Tags

3XFlag and HA

Promoter

ANF promoter

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miniDi-P2A-mCherry

Plasmid

#204358

Purpose

AAV vector for miniDi expression under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDi-P2A-mCherry

Use

AAV

Tags

mCherry

Mutation

The third intracellular loop (ICL3) of hM4Di was …

Promoter

hSyn

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-Ef1a-Puro-moxGFP

Plasmid

#227273

Purpose

AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI3sub-Kv-WPRE

Plasmid

#246616

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI3sub in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3sub-Kv

Use

AAV and Cre/Lox

Tags

Kv

Expression

Mammalian

Promoter

EF1a

Available Since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miniDq-P2A-mCherry

Plasmid

#204357

Purpose

AAV vector for miniDq expression under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDq-P2A-mCherry

Use

AAV

Tags

mCherry

Mutation

The third intracellular loop (ICL3) of hM3Dq was …

Promoter

hSyn

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8s-WPRE

Plasmid

#162374

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ERT2-iCre-ERT2

Plasmid

#178059

Purpose

Conditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.

Depositor

Edward Boyden

Article

Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

ERT2-iCre-ERT2

Use

AAV

Expression

Mammalian

Promoter

Human synapsin 1 (Syn) promoter

Available Since

Oct. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-fDIO-stGtACR2-FusionRed

Plasmid

#217975

Purpose

AAV transfer plasmid to express stGtACR2-FusionRed in a frt-dependent manner

Depositor

Edward Callaway

Article

Kirchgessner et al Curr Biol. 2021 Dec 6;31(23):5121-5137.e

Insert

stGtACR2-FusionRed

Use

AAV

Tags

FusionRed

Promoter

EF1a

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hM4D(Gi)-mCherry

Plasmid

#44362

Purpose

Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

Depositor

Bryan Roth

Article

Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

hM4D(Gi)-mCherry

Use

AAV; Adeno associated viral vector

Tags

mCherry

Promoter

human Synapsin 1

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

Plasmid

#119723

Purpose

AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Thomas Oertner

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Inserts

CaMPARI2
CaMPARI2

Use

AAV

Tags

NES-his

Expression

Mammalian

Mutation

F391W, L398V

Promoter

hSyn1

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-iGABASnFR2-WPRE

Plasmid

#218883

Purpose

AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Insert

iGABASnFR2

Use

AAV

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

CAG

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-eGFP-WPRE

Plasmid

#203843

Purpose

Flp-dependent EGFP expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

EGFP

Use

AAV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gThy1-CBh-mCherry-SV40late

Plasmid

#249126

Purpose

expression of gThy1 and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gThy1 (Thy1 Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-mCherry

Plasmid

#137132

Purpose

Intersectional viral expression of mCherry in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVLINKv1-pCALM1-Cre-3'-CHD1-FLAG

Plasmid

#244347

Purpose

3' AAVLINK plasmid for CHD1 expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

CHD1 (CHD1 Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

Plasmid

#159908

Purpose

Mutagenesis of Htr2a

Depositor

Larry Zweifel

Insert

Htr2a (Htr2a Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-tdTomato-WPRE

Plasmid

#51505

Purpose

Can be used to generate AAV virus that will express tdTomato in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdTomato

Use

AAV

Promoter

phSyn1

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]

Plasmid

#128589

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV8

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato (codon diversified version)

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

Syn

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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