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PurposeVector expressed CasRx and sgRNA_Ptbp1 for AAV package
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154001 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Vector typeMouse Targeting, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCasRx, Ptbp1 sgRNAs
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Alt nameNLS-RfxCas13d-NLS
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SpeciesM. musculus (mouse), Synthetic
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Insert Size (bp)3849
- Promoter GFAP, U6
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
INSERT 1: CasRx driven by GFAP promoter.
INSERT 2: DR-SgRNA_Ptbp1 driven by U6 promoter.
Ptbp1 gRNA 5: 5′-tgtagatgggctgtccacgaagcactggcg-3′
Ptbp1 gRNA 6: 5′-gcttggagaagtcgatgcgcagcgtgcagc-3′
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR was a gift from Hui Yang (Addgene plasmid # 154001 ; http://n2t.net/addgene:154001 ; RRID:Addgene_154001) -
For your References section:
Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice. Zhou H, Su J, Hu X, Zhou C, Li H, Chen Z, Xiao Q, Wang B, Wu W, Sun Y, Zhou Y, Tang C, Liu F, Wang L, Feng C, Liu M, Li S, Zhang Y, Xu H, Yao H, Shi L, Yang H. Cell. 2020 Apr 30;181(3):590-603.e16. doi: 10.1016/j.cell.2020.03.024. Epub 2020 Apr 8. 10.1016/j.cell.2020.03.024 PubMed 32272060