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Plasmid#188319PurposeExpression of LgBiT via sigma 70 promoter in E. coliDepositorInsertLgBiT
UseLuciferase and Synthetic BiologyTagsExpressionBacterialMutationPromotersigma 70 promoterAvailable sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
LgBiT-ST
Plasmid#207633PurposeA plasmid encoding LgBiT fused to SpyTagDepositorInsertLgBiT-SpyTag
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
LgBit-NbALFA
Plasmid#201484PurposeNanoBit detection of ALFA-tagged proteinDepositorInsertLgBit-NbALFA
UseTagsLgBitExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
NbV5-LgBit
Plasmid#201475PurposeNanoBit detection of V5-tagged proteinDepositorInsertNbV5-LgBit
UseTagsLgBitExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
LgBiT-hACE2
Plasmid#173431Purposeprotein expression plasmid of LgBiT-hACE2-IgG1 FcDepositorInsertLgBiT-hACE2-IgG1 Fc (ACE2 Human)
UseLuciferaseTagsIgG1ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LgBit-NbV5
Plasmid#201476PurposeNanoBit detection of V5-tagged proteinDepositorInsertLgBit-NbV5
UseTagsLgBitExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
NbAlfa-LgBit
Plasmid#201483PurposeNanoBit detection of ALFA-tagged proteinDepositorInsertNbALFA-LgBit
UseTagsLgBitExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
RC(WW)-LgBit
Plasmid#208931PurposeRecognition domain for in vitro MAPK-activity sensorDepositorInsertWW domain of PIN1 (PIN1 Human)
UseTagsHISx6, LgBit, and MBPExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
RC(14-3-3ε)-LgBit
Plasmid#208926PurposeRecognition domain for in vitro RSK-activity sensorDepositorInsert14-3-3ε (YWHAQ Human)
UseTagsHISx6, LgBit, and MBPExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-LgBiT
Plasmid#216779PurposeExpresses LgBiT in bacteriaDepositorInsertLgBiT
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LgBiT-SpyCatcher
Plasmid#223634PurposeProtein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis6, LgBiTExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FRB-LgBiT
Plasmid#182650PurposeFRB-LgBiTDepositorInsertFRB-LgBiT fusion
UseTagsLgBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hACE2-LgBiT
Plasmid#215837PurposeExpresses hACE2 receptor with LgBiT tag in mammalian cellsDepositorInserthACE2-LgBiT (ACE2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
NLS-LgBiT-FRB
Plasmid#183403PurposeExpresses nuclear-localized LgBiT-FRBDepositorInsertLgBiT-FRB
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-LgBiT-CaM
Plasmid#183401PurposeExpresses nuclear-localized LgBiT-CaMDepositorInsertLgBiT-CaM
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PDL1-LgBiT
Plasmid#223619PurposeLentiviral expression of human PD-L1 with LgBiT tag in mammalian cellsDepositorInsertPD-L1 (CD274 Human)
UseLentiviral; mammalian expressionTagsLgBiTExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD28-LgBiT
Plasmid#223616PurposeLentiviral expression of human CD28 with LgBiT tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsLgBiTExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NLS-LgBiT-GAI
Plasmid#183405PurposeExpresses nuclear-localized LgBiT-GAIDepositorInsertLgBiT-GAI
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQC.Flip-nluc(LgBiT1-8)CP[TEV]
Plasmid#200122PurposeRetroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cellsDepositorInsertFlip-nluc(LgBiT1-8)CP[TEV] T2A mCherry
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MBP-RGG-LgBit-RGG
Plasmid#176643PurposeLgBit fused to LAF-1 RGG domain with N-terminal MBP tagDepositorInsertMBP-RGG-LgBit-RGG
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only