pcDNA3.1-hACE2-LgBiT
(Plasmid
#215837)
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PurposeExpresses hACE2 receptor with LgBiT tag in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215837 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5368
- Total vector size (bp) 8257
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-hACE2-LgBiT was a gift from Thomas Gallagher (Addgene plasmid # 215837 ; http://n2t.net/addgene:215837 ; RRID:Addgene_215837) -
For your References section:
Dynamics of SARS-CoV-2 Spike Proteins in Cell Entry: Control Elements in the Amino-Terminal Domains. Qing E, Kicmal T, Kumar B, Hawkins GM, Timm E, Perlman S, Gallagher T. mBio. 2021 Aug 31;12(4):e0159021. doi: 10.1128/mBio.01590-21. Epub 2021 Aug 3. 10.1128/mBio.01590-21 PubMed 34340537