A highly sensitive NanoLuc-based protease biosensor for detecting apoptosis and SARS-CoV-2 infection.
Arakawa M, Yoshida A, Okamura S, Ebina H, Morita E
Sci Rep. 2023 Jan 31;13(1):1753. doi: 10.1038/s41598-023-28984-4.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
200119 | pCAG.CASP3-Myc | Expresses C-terminal Myc-tagged caspase 3 in mammalian cells |
200120 | pCAG.Myc-SARS2_3CL | Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL in mammalian cells |
200121 | pCAG.Myc-SARS2_3CL(C145A) | Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL C145A in mammalian cells |
200122 | pQC.Flip-nluc(LgBiT1-8)CP[TEV] | Retroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cells |
200123 | pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-Fluc | Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A] protease reporter and P2A firefly luciferase in mammalian cells |
200124 | pQC.Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)]-Fluc | Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] protease reporter and P2A firefly luciferase in mammalian cells |
200125 | pQC.Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc | Retroviral expression of Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells |
200126 | pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc | Retroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells |
200127 | pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)]-Fluc | Retroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)] protease reporter and P2A firefly luciferase in mammalian cells |
200128 | pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)]-Fluc | Retroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)] protease reporter and P2A firefly luciferase in mammalian cells |