pCAG.Myc-SARS2_3CL
(Plasmid
#200120)
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PurposeExpresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200120 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG-Myc
- Backbone size w/o insert (bp) 4872
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2 3CL protease
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SpeciesSARS-CoV-2
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Insert Size (bp)926
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Entrez GeneORF1ab (a.k.a. GU280_gp01)
- Promoter CAG
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG.Myc-SARS2_3CL was a gift from Eiji Morita (Addgene plasmid # 200120 ; http://n2t.net/addgene:200120 ; RRID:Addgene_200120) -
For your References section:
A highly sensitive NanoLuc-based protease biosensor for detecting apoptosis and SARS-CoV-2 infection. Arakawa M, Yoshida A, Okamura S, Ebina H, Morita E. Sci Rep. 2023 Jan 31;13(1):1753. doi: 10.1038/s41598-023-28984-4. 10.1038/s41598-023-28984-4 PubMed 36720982