RC(14-3-3ε)-LgBit
(Plasmid
#208926)
-
PurposeRecognition domain for in vitro RSK-activity sensor
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 208926 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepet-MBP
-
Backbone manufacturerAttila Reményi lab
- Backbone size w/o insert (bp) 4730
- Total vector size (bp) 5984
-
Modifications to backboneInsertion of LgBit fragment with NotI and XhoI, and then insertion of 14-3-3E with BamHI and NotI
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert name14-3-3ε
-
SpeciesH. sapiens (human)
-
Insert Size (bp)755
-
GenBank IDNM_006761
-
Entrez GeneYWHAQ (a.k.a. 14-3-3, 1C5, HS1)
- Promoter T7
-
Tags
/ Fusion Proteins
- MBP (N terminal on backbone)
- LgBit (C terminal on insert)
- HISx6 (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI, XhoI (not destroyed)
- 5′ sequencing primer malE
- 3′ sequencing primer T7_term (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
RC(14-3-3ε)-LgBit was a gift from Attila Reményi (Addgene plasmid # 208926 ; http://n2t.net/addgene:208926 ; RRID:Addgene_208926) -
For your References section:
Phosphorylation-Assisted Luciferase Complementation Assay Designed to Monitor Kinase Activity and Kinase-Domain-Mediated Protein-Protein Binding. Poti AL, Denes L, Papp K, Bato C, Banoczi Z, Remenyi A, Alexa A. Int J Mol Sci. 2023 Oct 3;24(19):14854. doi: 10.3390/ijms241914854. 10.3390/ijms241914854 PubMed 37834301