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Incorporation and Assembly of a Light-Emitting Enzymatic Reaction into Model Protein Condensates.

Guan M, Garabedian MV, Leutenegger M, Schuster BS, Good MC, Hammer DA
Biochemistry. 2021 Oct 14. doi: 10.1021/acs.biochem.1c00373. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
176642RGG-NanoLuc-RGGNanoLuc enzyme fused to LAF-1 RGG domain
176643MBP-RGG-LgBit-RGGLgBit fused to LAF-1 RGG domain with N-terminal MBP tag
176644MBP-RGG-SmBit-RGGSmBit fused to LAF-1 RGG domain with N-terminal MBP tag
176645NanoLuc-SZ2NanoLuc enzyme linked with SynZip2
176646SZ1-GST-mCherry-RGGSynZip1 linked surfactant protein that localizes to RGG condensate interface

Antibodies from Article