Incorporation and Assembly of a Light-Emitting Enzymatic Reaction into Model Protein Condensates.
Guan M, Garabedian MV, Leutenegger M, Schuster BS, Good MC, Hammer DA
Biochemistry. 2021 Oct 14. doi: 10.1021/acs.biochem.1c00373.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
176642 | RGG-NanoLuc-RGG | NanoLuc enzyme fused to LAF-1 RGG domain |
176643 | MBP-RGG-LgBit-RGG | LgBit fused to LAF-1 RGG domain with N-terminal MBP tag |
176644 | MBP-RGG-SmBit-RGG | SmBit fused to LAF-1 RGG domain with N-terminal MBP tag |
176645 | NanoLuc-SZ2 | NanoLuc enzyme linked with SynZip2 |
176646 | SZ1-GST-mCherry-RGG | SynZip1 linked surfactant protein that localizes to RGG condensate interface |