LgBiT-ST
(Plasmid
#207633)
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PurposeA plasmid encoding LgBiT fused to SpyTag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207633 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-29
- Backbone size w/o insert (bp) 5211
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLgBiT-SpyTag
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SpeciesSynthetic
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Insert Size (bp)573
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LgBiT-ST was a gift from Cole DeForest (Addgene plasmid # 207633 ; http://n2t.net/addgene:207633 ; RRID:Addgene_207633) -
For your References section:
Spatiotemporal functional assembly of split protein pairs through a light-activated SpyLigation. Ruskowitz ER, Munoz-Robles BG, Strange AC, Butcher CH, Kurniawan S, Filteau JR, DeForest CA. Nat Chem. 2023 May;15(5):694-704. doi: 10.1038/s41557-023-01152-x. Epub 2023 Apr 17. 10.1038/s41557-023-01152-x PubMed 37069270