...Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...Inventory Page . Don’t See What You’re Looking For? Please (Link opens in a new window) let us know what you...you would like to see available in viral format. Please note this does not guarantee viral service, but...

...Plasmids and Resources Addgene is working with the leading scientists in the field to assemble the reagents...links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial immune ...visualize genomic loci. dCas9-FokI dCas9 fused to FokI nuclease generates a DSB when expressed with two gRNAs....

...designed to be used with any CRISPR nuclease and function. Please see the publication or plasmid information...and maintained by the Doudna Lab . gRNA Plasmids Please note that the following table lists both published...

...Looking For? Please let us know what you would like to see available in viral format. Please note this does... . Note: For a list of all 100 µL sized sample, please see our Control AAV Inventory . The catalog ID ...

...information on ordering from a for-profit entity, please see our Plasmid Distribution to Industry page. ...you describe the use of a plasmid in an article, please fill out this Google Form and we will add your ...resources, such as reagents, data, news, and more, please see Ginkgo's Synthetic Biology (and beyond) Community...

...Looking For? Please let us know what you would like to see available in viral format. Please note this does...in trans . (3) Nascent G-protein coated RAVB is released from the starter cell and free to infect neighboring...

...Learn about the Cre-Lox system and how it can be used for genetic rearrangements and recombination. Use...Zeng 140135 pAAV-EF1a-iCreV Split Cre fused to nuclear localized wild-type VVD EF-1 alpha AAV Zeng 140284...expression of Cre Mouse Sncg AAV Hu 154018 LiOn-CMV∞Cre leak-proof iOn (LiOn) Cre switch CMV Mammalian Livet ...containing FLEx technology. Read the genOway press release for more information. Empty backbones to regulate...24334 hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean Heat-inducible reporter with Cre dependent H2B-RFP...

...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...enter the nucleus during mitotic breakdown of the nuclear envelope). Additionally, γ-retroviruses integrate...integrate randomly into the host's genome, which can lead to oncogenesis (termed insertional mutagenesis). ...

..., additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can...or Cas9 domains PE7 - addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA...

...biology - deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for...Plant Base Editors Save Time with Transient Plant Leaf Transformations RNA Interference in Plant Biology...

...site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make...Highlights of Zebrafish Tools in our Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR...

...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan...

...Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate double-stranded breaks ( DSBs...Suggestions Don’t see what you are looking for? Please let us know what you would like to see available... Suggestion Form (Link opens in a new window) . Please note this does not guarantee viral service, but...

...plasmids The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun...and Sox2 Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Cell Stem...Baldwin Fibroblasts iTSCs Lentiviral Mouse Extensive Nuclear Reprogramming Underlies Lineage Conversion into...

...Structural Genomics Consortium (SGC) Plasmids Opher Gileadi Lab plasmids Haian Fu Lab plasmids The TREAT-AD...AD and creating high-quality, well-characterized lead molecules for prioritized targets that are valued...

... method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease fusions...

...these vectors also have a FLAG epitope tag and nuclear localization signal at their amino-terminus. The...Assembly TALEN Kit . Vectors encoding wild-type FokI nuclease domains ( Sander et al., Nat Biotechnol. 2011 ...

...plasmids required to practice CRISPR-Cas/RNA-Guided Nuclease ( RGN ) technology in zebrafish. The Joung Lab...expression of a transcript encoding the Cas9 endonuclease. Details for producing mRNA suitable for expression...

...plasmids required to practice CRISPR-Cas/RNA-Guided Nuclease ( RGN ) technology in human cells. The Cas9 expression...expression of a transcript encoding the Cas9 endonuclease. Both vectors also express a C-terminal 3X FLAG...

...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ...fluorescent protein plasmids. Use this guide to learn more about the many applications of fluorescent ...