... and guides to help you use CRISPR in your lab. Learn More Guide to CRISPR technology Addgene Blog Posts...sequence) can be provided, to compare the predicted cleavage position to the position of the observed mutations... the Chan Zuckerberg Biohub . gRNA Design Deep learning models to design gRNAs and predict their efficiency...sequence or list of sequences. RGEN (RNA-guided endonuclease) Tools: Cas-OFFinder & Cas-Designer From the...Targeter Originally developed to identify zinc finger nuclease sites, this tool from the Joung Lab has been expanded...

...codon-optimized Cas9 nuclease expression plasmid. The arrangement of the Nuclear Localization Signal (...optimized for the most efficient localization of the nuclease. This optimized hCas9 expression has been shown...expression plasmid, specifically designed for efficient nuclear localization in eukaryotes....

...Browse Addgene's collection of KLF plasmids and learn more about the scientists who have created and shared... Krüppel-like factors (KLFs) are a family of at least 17 DNA binding transcriptional regulators with diverse...collaborative efforts within the KLF community have lead to the establishment of this shared resource on ...encourage you to contribute to this community resource. Learn about the benefits of depositing plasmids with Addgene...

...downstream of the sequence to be cleaved. This non-specific cleavage is thought to activate programmed...DNA. In bacteria, once they have recognized and cleaved the target RNA sequence, they adopt an enzymatically...enzymatically active state and can bind and cleave additional RNAs regardless of homology to the crRNA. This...

...chiRNAs can induce defined deletions between the two cleavage sites, while the addition of an ssODN donor template... of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue... 46294 pBS-Hsp70-Cas9 : A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.... 45945 pHsp70-Cas9 : The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter...

...Hochschild and colleagues ( Dove et al., Nature 1997 ) and later modified by Pabo and colleagues ( Joung et...this Kit These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite...

...criteria. Please consider using fewer filtering options. Don’t See What You’re Looking For? Please let us...Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag...you would like to see available in viral format. Please note this does not guarantee viral service, but...

...Lab on the antibody webpage. To learn more about these antibodies, please visit the Institute for Protein...mammalian cells. To learn which specific QC experiments were performed on your lot, please email [email protected]...

...reports have shown that engineered zinc finger nucleases (ZFNs) are effective tools for making targeted...the spacer sequence between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT...open-source' engineering of customized zinc-finger nucleases for highly efficient gene modification. Maeder...mutagenesis in zebrafish using customized zinc-finger nucleases. Foley JE et al. Nat Protoc. . 2009. Dec 3. 4(...

...pCS2plus-mCerulean 156-239-GGGS-TDP-43 TARDBP mCerulean CMV ALS Marco Morsch 162618 pCS2plus-mCerulean 156-...Parkinson's, FTD Michael Davidson 55431 mCerulean3-MAPTau-C-10 MAPT mCerulean3 CMV Parkinson's, FTD Michael Davidson...Sidhu 110061 pDUET-1-alpha-synuclein-mCerulean3-His6 SNCA His, mCerulean3 T7 Parkinson's Dan Mulvihill 110486...Lippincott-Schwartz 164214 PLEX-PGK-ANXA11-mCerulean ANXA11 mCerulean PGK ALS Jennifer Lippincott-Schwartz ...mRuby CMV ALS Jennifer Lee 216227 TDP-43 mCerulean TARDBP mCerulean CMV ALS Jennifer Lee 216843 pET-Duet_...If you do not see a particular gene of interest, please search our full plasmid collection using the search...Bacterial Yeast Other Promoter CMV T7 polH GAL Other Clear Filters Addgene ID Plasmid Name Gene Tags Promoter...

...Which Type of Virus Should I Use? Use this guide to learn more about the different types of viruses that are...select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit ...viruses are temporary in the host. Use this guide to learn more about the different types of viruses that are...depending on the insert type and envelope used. Please consult with your institution’s Biosafety Committee...

...Deisseroth AV-1-PV3248 105557-AAV1 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG James M. Wilson AV-1-PV3508 98928-AAV1 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...Wickersham AV-9-PV3248 105557-AAV9 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG James M. Wilson Cre Recombinase AV-1-PV1090...Plasmids are available for all the items listed below. Please let us know (Link opens in a new window) what you...you would like to see available in viral format. Please note this does not guarantee viral service, but...pENN.AAV.CB7.CI.TurboRFP.WPRE.RBG James M. Wilson Please contact [email protected] with any questions. Return...

...pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity ...DNA-binding domain of a TALE, along with strong nuclear localisation tags and repress transcription initiation... BioSciences (N153AA, C63AA) and showed robust cleavage activity in several later studies. pCAG-T7-TALEN...

...- Insect expression vector from Gileadi lab Xenopus SP6 for transcription and injection...full Fluorescent Protein Guide NLS Nuclear Localization pENTR4-myc-nuc - pEntry ... vector that adds a C-terminal nuclear localization signal FNpcDNA3 - N-terminal ...N-terminal FLAG and SV40 nuclear localization signal Myr Membrane Localization pWZL-Neo-Myr-Flag-DEST...

...12targeting sgRNA. Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase...publication. For questions regarding the protocol please contact Dr. John Calarco: [email protected]...

... Design Tools CRISPR Blog Posts Assorted Cas9 nucleases from different bacteria as well as a mammalian...described in: A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity. Jinek M, Chylinski...structure-specific RNA processing by a CRISPR endonuclease. Haurwitz RE, Jinek M, Wiedenheft B, Zhou K,...

...work in the model plant Nicotiana benthamiana . Please refer to the Supplementary methods section of the... Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nekrasov V, Staskawicz B, Weigel D, Jones JD...pK7WGF2::hCas9 : Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with ...

...hap-16.48 promoter. Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase...optimized for C. elegans expression, flanked by two nuclear localization signals. pMB62 and pMB63 drive expression...

...new window) (Penn Vector Core) and Addgene are pleased to announce a partnership to provide high-quality...Suggestions Don’t see what you are looking for? Please let us know what you would like to see available...filling out our Viral Service Suggestion Form . Please note this does not guarantee viral service, but...

...Plasmids and Resources Addgene is working with the leading scientists in the field to assemble the reagents...links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial immune ...visualize genomic loci. dCas9-FokI dCas9 fused to FokI nuclease generates a DSB when expressed with two gRNAs....