How to Design Your gRNA for CRISPR Genome Editing
Type
Blog Post
Published
Sept. 24, 2020, 1:15 p.m.
...nature of non-homologous end joining (NHEJ) often leads to the generation of indels and thus frameshifts...activation) and CRISPRi (inhibition) technologies – a nuclease-dead Cas9 (dCas9) is directed near the promoter...landscape of mismatches that can nevertheless still lead to activity has been established, and can be used...that the consequences of off-target activity, at least for the experimental conditions used, led to no ...the generation of truly isogenic cell lines than nuclease-mediated off-target effects.” Further, large-scale...al., 2019). Again, the experimental strategy is clear: for any gene of interest, one should require that... Liu DR (2020) Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors....