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Showing: 661 - 680 of 819 results

  1. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...appear as opaque dots (colonies should contain at least 50 cells and be well-separated. If the growth rate...to a 96-well assay plate. We generally freeze at least 2 ampules each of 4 clones with different levels...

  4. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...cell level as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness...selectively is especially essential. If you want to learn more about how viruses can be used for chemigenetics...

  5. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...used to: Monitor dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle...PMC4831023. Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation Catch...

  6. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery. Cas9 ... Blog Check out our CRISPR Featured Topic Page Learn about CRISPR Delivery Using Ribonucleoproteins (...

  7. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...temperature). Photostability: Fluorescent molecules gets bleached (i.e. lose the ability to emit light) after prolonged...imaging technologies to study gene expression, nuclear localization, and dynamics” Curr. Opin. Cell. Biol...

  8. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...called a standard. The ideal standard curve has at least five dilutions, with each step of the series diluted...good” is my standard curve? A poor standard curve leads to over or underestimation of an unknown sample’...

  9. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...After uncoating, reverse transcription follows the release of the two strands of viral RNA and of the viral...genome can direct the production, packaging, and release of progeny viral particles. And there you have ...

  10. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...target the CNS or the PNS, respectively.  Read on to learn more about these two new AAV capsids and how they... Blog Important considerations when using AAVs Learn more about using AAV as a tool for gene expression...

  12. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene expression in...development. As a result, Luk Vandenberghe and colleagues at MEEI co-founded a non-profit to help push ...

  14. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...step may not be necessary. In fact, you may get cleaner results by not permeabilizing as you’ll prevent...interacts with cholesterol in cell membranes and leaves many membrane-associated proteins in place. In ...

  15. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag (derived from the tyrosine-protein...on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids ...

  16. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...antimicrobials using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed... Bikard, David, et al. “Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials.” ...

  17. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 2:15 p.m.
    ...embryogenesis (Figure 3). mScarlet-I3 also offered clearer signals due to reduced background signal when tested... gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain carrying Cas9 to allow the introduction...

  18. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...containing glucose and L-arabinose at 37oC for at least 12 hours, or at lower temperatures for longer. Cultures... induction of expression… but longer expression leads to greater amount of gas vesicles and increased ...

  19. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ... viral vector preparation protocol, and we are pleased to report that over 95% of viral capsids in representative...Resources on Addgene.org Find All Available AAV Learn More about Virus Production Find Viral Vector Protocols...

  20. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations...understand isoform-specific gene regulation — at least, more straightforward than generating cDNA for each...
Showing: 661 - 680 of 819 results