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Showing: 641 - 660 of 819 results
  1. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org...
  2. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...required the incorporation of a cleavage site and a post-purification cleavage step to remove the tag in order...
  3. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...Treatment of the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, ...PMC4097317. Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent Proteins...
  4. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect, perhaps...give advice for your interviews.  Pro tip: You can learn a lot from the company LinkedIn page. This can include...
  5. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...reprogramming, and beyond. For further details, please check out Wong et al 2016 and Wong et al 2015. ...PMCID: PMC4780610. Resources on the Addgene Blog Learn Best Practices for Designing Your gRNA Read How ...
  6. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...photoconvertible variants, it seems like GFP is always learning new things. Now, work from Connie Cepko’s lab ...easier. Advantages and other possibilities It’s clear that Cre-DOG and T-DOGGs open up many new possibilities...
  7. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...characterize the transcriptome. The first data release for CeNGEN is planned for August 15th, 2019. For...PMC4689222. Additional resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. ...
  8. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...depend on many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed gRNA...choosing a MoClo strategy and kit if you want to learn more about this cloning technique! Many thanks to...
  9. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...different generations of lentiviral packaging systems please see Addgene’s Lentiviral Guide. While the third...cell line. For more tips on lentiviral titering please see our blog post. Between choosing the best lentiviral...
  10. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ... engineered mice in your experiment. As you’ve learned in Part 1, there are many types of genetically ...about Cre-lox Read our Genome Engineering Guide Learn more in our CRIPSR guide ...
  11. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ... cells (Sustarsic and Kapanidis 2015), which may lead to monitoring of these processes in live animals...Addgene Blog Tips for using FRET in Your Experiments Learn about Fluorophore Targeting for FRET Studies FRET...
  12. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...oligos to arrive. We also were careful to set up clear tasks for each member of the team to accomplish ...Lab Member and Sample 6 Cofounder Michael Koeris Learn How Minigenomes Are Being Used to Study Dangerous...
  13. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...behaviors, and a chronic feeling of hunger that can lead to excessive eating and obesity. What is fascinating... to visualize changes in gene expression and may lead to the development of a whole new range of methods...
  14. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ... to admit to inviting interesting friends and colleagues to events for this purpose. If possible, make... more than 40 minutes for the panel discussion leaving 20 minutes for audience questions (30 if discussion...
  15. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...AAV5. AAV9 preps and more AAV1 versions will be released soon. Visualize axonal projections and putative...
Showing: 641 - 660 of 819 results