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Showing: 641 - 660 of 797 results

  1. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...characterize the transcriptome. The first data release for CeNGEN is planned for August 15th, 2019. For...PMC4689222. Additional resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. ...

  2. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...depend on many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed gRNA...choosing a MoClo strategy and kit if you want to learn more about this cloning technique! Many thanks to...

  3. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ... cells (Sustarsic and Kapanidis 2015), which may lead to monitoring of these processes in live animals...Addgene Blog Tips for using FRET in Your Experiments Learn about Fluorophore Targeting for FRET Studies FRET...

  4. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...different generations of lentiviral packaging systems please see Addgene’s Lentiviral Guide. While the third...cell line. For more tips on lentiviral titering please see our blog post. Between choosing the best lentiviral...

  8. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...antimicrobials using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed... Bikard, David, et al. “Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials.” ...

  9. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...behaviors, and a chronic feeling of hunger that can lead to excessive eating and obesity. What is fascinating... to visualize changes in gene expression and may lead to the development of a whole new range of methods...

  10. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ... to admit to inviting interesting friends and colleagues to events for this purpose. If possible, make... more than 40 minutes for the panel discussion leaving 20 minutes for audience questions (30 if discussion...

  11. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...AAV5. AAV9 preps and more AAV1 versions will be released soon. Visualize axonal projections and putative...

  12. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...cell level as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness...selectively is especially essential. If you want to learn more about how viruses can be used for chemigenetics...

  13. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...appear as opaque dots (colonies should contain at least 50 cells and be well-separated. If the growth rate...to a 96-well assay plate. We generally freeze at least 2 ampules each of 4 clones with different levels...

  16. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...used to: Monitor dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle...PMC4831023. Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation Catch...

  17. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery. Cas9 ... Blog Check out our CRISPR Featured Topic Page Learn about CRISPR Delivery Using Ribonucleoproteins (...

  18. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...temperature). Photostability: Fluorescent molecules gets bleached (i.e. lose the ability to emit light) after prolonged...imaging technologies to study gene expression, nuclear localization, and dynamics” Curr. Opin. Cell. Biol...

  19. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag (derived from the tyrosine-protein...on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids ...
Showing: 641 - 660 of 797 results