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  1. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...Biochemistry or International Finance. A book club could lead to smart, sometimes contentious, discussions. I ...comments! Additional resources on the Addgene blog Learn about company culture at Addgene Read about other...

  2. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ... over 6,500 requests for these plasmids. While clearly an outlier, this story had us wondering: is there...journals gets more citations and is more likely to lead to an Addgene deposit, since scientists may believe...

  3. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter activity persisted...with other pathways. They also reduced system leakiness and increased dynamic range to around 100-fold...

  4. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ... is now a common feature in reporter plasmids.  Learn How Luciferase Can Be Used In Concert with Fluorecent...Post-translational Modification Not Required Required Photobleaching/Phototoxicity Not susceptible Susceptible ...

  5. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ... convince the PI to choose me?” We asked three leading professors how they hire scientists for their labs... Choose a lab that brings on the happy dance at least once in awhile. Acknowledgments Thank you to Dr...

  6. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...transfection process is not integrated into the nuclear genome, in the absence of plasmid replication, ... lines expressing the Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T antigen (...

  7. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA. Find pSpCas9n(BB)-2A-GFP (PX461). pSpCas9(...CRISPR collection Find plasmids for your research  Learn about our viral service ...

  10. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...consider them worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation To resolve this impasse...potential collaborators and interested audiences. Please visit our team wiki or contact us at igemiiserb...

  11. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ... canonical 20 amino acids. But in your quest to learn more about your protein of interest, you may find... both translation and fidelity of translation, leading to system-wide effects. And on top of that you ...

  12. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...retrograde AAV work are not yet understood, one thing is clear: this application opens a lot of doors in neuroscience...Resources on the Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's...

  13. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation of ...example of how sharing of data and molecular tools can lead to faster and more efficient science. Researchers...

  14. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...involved yourself. You probably won’t be surprised to learn that Addgene’s mission statement extends beyond ...and our graphic designer to make sure things are clear, accurate, and in line with Addgene style.    ...

  15. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... tail vein injections but would like to avoid "leaking" into the brain, would you recommend using the ...for very high MOI on top of very high coverage. Please see this paper for more information about PHP.eB...

  18. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...two Jack Russell Terriers. More SynBio Reading: Learn more about Revolution Bioengineering Follow RevBio...

  19. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...selection markers.  The yeast origin of replication (Please note: This first section primarily pertains to ... used for color and growth selection if divalent lead ions are used in the growth media. ura4+ pyrimidine...
Showing: 621 - 640 of 819 results