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Showing: 621 - 640 of 797 results

  2. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation of ...example of how sharing of data and molecular tools can lead to faster and more efficient science. Researchers...

  3. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...involved yourself. You probably won’t be surprised to learn that Addgene’s mission statement extends beyond ...and our graphic designer to make sure things are clear, accurate, and in line with Addgene style.    ...

  4. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... tail vein injections but would like to avoid "leaking" into the brain, would you recommend using the ...for very high MOI on top of very high coverage. Please see this paper for more information about PHP.eB...

  5. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA. Find pSpCas9n(BB)-2A-GFP (PX461). pSpCas9(...CRISPR collection Find plasmids for your research  Learn about our viral service ...

  9. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...reprogramming, and beyond. For further details, please check out Wong et al 2016 and Wong et al 2015. ...PMCID: PMC4780610. Resources on the Addgene Blog Learn Best Practices for Designing Your gRNA Read How ...

  10. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...two Jack Russell Terriers. More SynBio Reading: Learn more about Revolution Bioengineering Follow RevBio...

  11. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...selection markers.  The yeast origin of replication (Please note: This first section primarily pertains to ... used for color and growth selection if divalent lead ions are used in the growth media. ura4+ pyrimidine...

  12. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...photoconvertible variants, it seems like GFP is always learning new things. Now, work from Connie Cepko’s lab ...easier. Advantages and other possibilities It’s clear that Cre-DOG and T-DOGGs open up many new possibilities...

  13. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect, perhaps...give advice for your interviews.  Pro tip: You can learn a lot from the company LinkedIn page. This can include...

  14. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org...

  15. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...Treatment of the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, ...PMC4097317. Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent Proteins...

  16. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...required the incorporation of a cleavage site and a post-purification cleavage step to remove the tag in order...

  18. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...behaviors, and a chronic feeling of hunger that can lead to excessive eating and obesity. What is fascinating... to visualize changes in gene expression and may lead to the development of a whole new range of methods...

  19. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...oligos to arrive. We also were careful to set up clear tasks for each member of the team to accomplish ...Lab Member and Sample 6 Cofounder Michael Koeris Learn How Minigenomes Are Being Used to Study Dangerous...
Showing: 621 - 640 of 797 results