Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
Type
Blog Post
Published
May 10, 2016, 2:30 p.m.
...functional Cas9 nuclease, so there’s no potential for aberrant DNA cleavage. Direct HIV-1 cleavage allows T-...the HIV-1 genome from infected cells. Read on to learn how CRISPR can take on HIV-1 in vitro, and what ...immune system, notably CD4+ T-cells, and eventually leads to acquired immunodeficiency syndrome (AIDS) in ...that most of the HIV-1 genome had been excised, leaving only a small portion of the LTRs joined together...expression. A pooled analysis found no evidence of Cas9 cleavage at predicted off-target sites with up to 7 mismatches...which could result in higher rates of off-target cleavage.
In both cases, translating the in vitro work ...1 is limited to a subset of T-cells, but it is unclear if enough T-cells could be reached to ablate a ...