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Showing: 561 - 580 of 819 results

  1. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...very high titers (Zingg et al., 2017), which can lead to cells in far away brain regions expressing your...experiment. Some inducible constructs can exhibit leaky expression in the absence of the inducing drug, ... result in overexpression of your gene and could lead to toxicity or trouble interpreting your results...

  2. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...temperature 5-10 degrees above the primer and be at least 25 bp away from each primer, ideally located close...suitable for your cell lines and/or tissue types.  Please do note my oh-so-subtle use of reference genes,...can be used up quickly by abundant transcripts, leading to falsely lowered levels for less abundant transcripts...

  4. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...me: Drag-and-drop genome insertion, without DNA cleavage, with CRISPR-directed integrases🎵 On the 6th ...Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in of long sequences🎵On the...

  5. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...Guide to Synthetic Biology One of the best ways to learn about a new field is simply to know where to look...have additional resources that might be helpful, please reach out to me by email.  About the author Niko...

  6. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...this series, we’ll highlight one tip that we’ve learned in the lab that will hopefully make your life a...be able to locate materials quickly and easily. Learn more about using barcodes. Check out our “Lab...

  7. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ...all of the answers, but we’re betting that your colleagues do. That’s why we’re launching the AAV Data Hub...thank you for your contribution to open science.  Please note that the AAV Data Hub is a beta project, and...

  8. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ...’ll discuss the types of programs SITN runs and learn how they think about communicating science. Listen... our Interview with Science Rapper Tom McFadden Learn about SITN's work at DayCon 2015 5 Reasons to Use...

  9. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...really incredible science - I hope you've enjoyed learning about it (and maybe even picked up a new technique...a research story you want to share on our blog, please reach out to [email protected] - we'd love to hear...

  10. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...cleverness, and crafting skills have surpassed your colleagues. Success is sweet – as sweet as all that Halloween...yourself on in the coming days. Hopefully, I’ve made clear how excited we get for Halloween and how seriously...

  11. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ... (AAVrg), and volumes of 1 mL, 2.5 mL, or 5 mL. Please note that all orders will be shipped in 100 µL ...Request webpage, or our announcement blog post to learn more. And our helpful scientific support team is...

  12. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...Founders - Melina Fan, Ken Fan, and Benjie Chen. We learn what it was like to get Addgene off the ground and...idea got off the ground Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption ...

  13. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...shared might have you wondering how many plasmids leave Addgene headquarters every day. That would be an...celebration was so much fun! Here's the Addgene Leadership team in all their photo booth glory! #1millionplasmids...

  15. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...happy during their time in grad school. As you'll learn, grad school has its ups and downs for everyone ...Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve the ...

  17. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ... we stick to citation counts for a moment, it’s clear that articles in less-prestigious journals have ...citations to all articles, Anurag Acharya and colleagues report, the percentage of citations to articles...

  18. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ..., I drank 3-4 cups of coffee a day, and I had colleagues who were much more wired. Here at #SfN14 there...Addgene in their labs. And it has been exciting to learn about all the amazing research being done with plasmids...

  19. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...generated through your organization in this field. Please also enter the billing address for your organization...department requires a quote in order to generate a PO, learn more about how to create a quote. How do I pay ...

  20. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ... the Cas9-gRNA RNP complex via the fused HUH endonuclease PCV. This increases HDR efficiency up to 30-...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...
Showing: 561 - 580 of 819 results