... variety of expression systems. Keep reading to learn more about the Gateway cloning method and its advantages...conditions, the attL and attR sites can recombine, leading to the excision of the phage from the bacterial...Resources on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony...

...contributed by the Mesothelioma Cancer Alliance. We have learned much about the causes of cancer and the different...nucleus, if the cell dies from injury, this protein leaks out into the cytoplasm, migrates out of the cell...About Cancer and Addgene's Cancer Collection Pages Learn How the ClonTracer Library Can be Used to Study ...

..., using up valuable resources in the cell thus leading to slowed growth. Additionally, some protein products...well for most experiments, the lac promoter can “leak,” meaning that a low level of expression exists ...your favorite protein expression tips and tricks! Leave a comment below or browse Addgene’s collection of...

...subsequently changed fields and my cohort of friends and colleagues had moved on as well, so I knew very few people...people, it was certainly an issue worth addressing. Clear expectations have been established for future participants... after our initial pilot, and look to take some leaps. Binning Singletons is its own organization, working...

...been demonstrated in zebrafish using zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another...zebrafish zygote transforms into an organism with a clearly established body plan including head, eyes, and... tracked in whole brains of swimming, actively learning larvae, providing unprecedented views into cognitive...

...this gap by both highlighting that researchers do learn MANY transferable skills while working in the lab...to live in your lab, right? Wrong! While it is clearly a good idea to be diligent in your research, remember...career center. Alternatively, it might even be a colleague. A mentor does not necessarily need to be someone...

...CRISPR 101 Series Learn about the PAM Requirements of Different Cas9 variants Learn about CRISPR Pooled.... & Liu, D. R. Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors....

...much trial and error to learn the scripting language, but our path forward was clear: to build one process...assembly algorithms meant that we already had a clear idea of the necessary steps to perform:    ...

...for Titering Your Lentivirus Learn All About Viral Vector Elements Learn How to Do Genome-Wide Screens... target cells. Poor tissue culture practices can lead to low titers and poor transductions. Cell lines...

...a first time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an ..., would help improve split vector strategies and lead to greater and more consistent transgene expression...

...very high titers (Zingg et al., 2017), which can lead to cells in far away brain regions expressing your...experiment. Some inducible constructs can exhibit leaky expression in the absence of the inducing drug, ... result in overexpression of your gene and could lead to toxicity or trouble interpreting your results...

...temperature 5-10 degrees above the primer and be at least 25 bp away from each primer, ideally located close...suitable for your cell lines and/or tissue types.  Please do note my oh-so-subtle use of reference genes,...can be used up quickly by abundant transcripts, leading to falsely lowered levels for less abundant transcripts...

...episode of our Hot Plasmids podcast series, you'll learn about optogenetics tools for controlling protein...plasmids covered in this segment Hot Plasmid 1: Photocleaveable PhoCl protein for controlling molecular processes...

...me: Drag-and-drop genome insertion, without DNA cleavage, with CRISPR-directed integrases🎵 On the 6th ...Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in of long sequences🎵On the...

...Guide to Synthetic Biology One of the best ways to learn about a new field is simply to know where to look...have additional resources that might be helpful, please reach out to me by email.  About the author Niko...

...this series, we’ll highlight one tip that we’ve learned in the lab that will hopefully make your life a...be able to locate materials quickly and easily. Learn more about using barcodes. Check out our “Lab...

...’ll discuss the types of programs SITN runs and learn how they think about communicating science. Listen... our Interview with Science Rapper Tom McFadden Learn about SITN's work at DayCon 2015 5 Reasons to Use...

...all of the answers, but we’re betting that your colleagues do. That’s why we’re launching the AAV Data Hub...thank you for your contribution to open science.  Please note that the AAV Data Hub is a beta project, and...

...really incredible science - I hope you've enjoyed learning about it (and maybe even picked up a new technique...a research story you want to share on our blog, please reach out to [email protected] - we'd love to hear...

...cleverness, and crafting skills have surpassed your colleagues. Success is sweet – as sweet as all that Halloween...yourself on in the coming days. Hopefully, I’ve made clear how excited we get for Halloween and how seriously...