...your questions, data, and conclusions should be clearly identified and interpreted in less than a couple...to read. The key to the success of this style is clear borders around your columns, and good spacing between...used for displaying all of one’s figures, or at least the majority of the visual data. This central box...

...cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic potential...of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult...Early-stage trials showed a high success rate in clearing misfolded protein levels as measured in the blood...

...receptors hijack cell signaling pathways and can lead to such responses as decreased heart rate (Redfern...properties such as endogenous gene expression, which can lead to changes in normal cell physiology and function...posts about neuroscience Resources on addgene.org Learn more about chemogenetics in our chemogenetics guide...

...Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control...Self-complementary AAV mediates gene targeting and enhances endonuclease delivery for double-strand break repair." Gene...

...list. 7) Internships – Learn on the job Nothing prepares you better than learning on the job. Try to get...path you want to follow, check out the distance learning courses at the National Council for the Training...

...Washington DC; convening meetings of experts and leaders from academia, industry, community, education and...the biological sciences, as well as to acquire leadership and communication experience. These included ... science career path Resources on Addgene.org Learn how Addgene engages with the community on our Addgene...

...final recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide webpage, and references...purified plasmid stock should be analyzed by endonuclease restriction digest or fully sequenced to confirm...a week), and do NOT harvest the cells before at least 10 days of incubation as it will result in very ...

...one is in the making by Dr. Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated...these criteria and deemed positive, it will be released through the website for public reference. The ... sending antibodies, and/or working with Dr. Wu please contact us here! Ava Shipman is a research assistant...

...Kamiyama D. Commun Biol. 2021 Daichi Kamiyama Cerulean Cerulean(1-10) for use with GFP(11) Multiplexed labeling...ECFP C155 of ECFP pairs with N173 derived from Cerulean or Venus Identification of new fluorescent protein...

...Our Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has...Lineage Tracing Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral...

...account, business cards, incorporation, laboratory lease, etc. I still flip to that page in my lab notebook... into executing a successful business that the learning curve is pretty steep. But we can rely on one ... about this transition (so far) and what we’ve learned.  Thank you to our guest bloggers! Nikolai Braun...

...pooled library with Addgene, you may be surprised to learn that we ask for an amplification protocol with your... repeated amplifications of pooled libraries can lead to issues such as recombination and loss of plasmid...Barcode Watermelon Library 5,000,000 30 bp At least 200 ng Liquid Electroporation Protocol (DOCX...

...continue to to build and develop this new resource! Please leave us your thoughts in the comments section below...Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used in Cancer...

...conference If there’s a cool new method that you learned about at the talk, bring it to your next lab meeting...fruitful. After the conference Bring back what you’ve learned from the poster session to your lab. Perhaps some...scientific communication, and scientific networking Learn how to set up and informational interview and Expand...

... Resources on the Addgene Blog Learn about inducible promoters Learn about repressible promoters Check...the addition of post-translational modifications Learn more molecular biology in our Plasmids 101 eBook...

...FPs to highlight various subcellular structures. Please see Figure 1 for commonly used markers for major...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...Microscopy. Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous Gene Tagging ...

... in the primary literature, and as a result can leave scientists under-informed with respect to how different...the year, those that meet our minimum standards (clear and detailed methods and image descriptions, a minimum...computer files. If you asked your team members or colleagues: How much unpublished AAV data do you have sitting...

...fantastic model organism for many reasons, not the least of which is ease of transformation. There are many...finding true transformants more difficult. Don’t leave your plants on selective plates for too long: even...recovered from the stress of selection you can clip a leaf and extract genomic DNA for genotyping. This won...

...commonly result in a complete loss of function while nuclease-deactivated Cas9 (dCas9) can be tethered to a ...which contains multiple point mutations in the nuclease domain. These mutations result in an inactive ...about CRISPR Kinome Libraries available at Addgene Learn about the advantages of pooled CRISPR libraries ...

...retroviruses. Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing ...Third-generation plasmids can be packaged with either system. Please note that the generation used to package the virus...Addgene Blog: Download our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus...