...ever like to write about a recent plasmid deposit please sign up here.        New toolkit for N or C terminal...widely used genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for a specific...and follow the DNA sequence targeted by the Cas9 nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires...

...dysregulation. A targeted TF screening approach can not only lead to a better understanding of disease onset, but ...isoforms. Due to the size of the collection, we’re releasing individual plasmids in batches. If there’s something....2017.016. Additional Resources on the Addgene Blog Learn More about Lentivirus Figure out How to Get the ...

...results as soon as the gels ran.” Dr. Ellis tries to lead his team as a scientists and a friend. George Church...3 for tips on how to develop your role as a lab leader, and then on to Chapter 4 for advice on staffing... labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early Career Scientists...

...enter the nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses can transduce both dividing... small enough to enter the nucleus through the nuclear pore complex. This feature may be useful for the...stimulates the expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -R -U5 LTR...

... networking for scientists Learn why scientists should be on Twitter Learn about using Reddit for science...Developing science communication skills, while also learning about new scientific developments and helping ...

...This is just a primer so that you are aware of at least some of the possibilities. Basically, if you can...between pathogens and their hosts. It’s not always clear which side he roots for. You can try to convince...PMC3172133. Additional Resources on the Addgene Blog Learn about Using Cre in Drosophila Read Other Genome ...

...search tool updates. Now, let’s take a look at the least visible part behind these changes: the backend technology...engine! Additional resources from the Addgene blog Learn about other features on Addgene's website Find a...step-by-step guide to using Addgene's new search Read to learn more about why we redesigned our homepage and mascot...

... Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder, Melina Fan, we created...Production webpage). While the new team took the lead on operationalizing viral production and distribution...Out the Top Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral Service in Your Research...

...immune response. There are three main pathogen clearing functions or effector functions of the constant...are two IgA subclasses: IgA1 and IgA2.  You can learn more about isotype classes in our Antibodies 101...NBK27162/ Additional resources on the Addgene blog Learn about the advantages and disadvantages of using ...

...our Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one..., McPhee DA, Verity E, Dyer WB, Wesselingh SL, Learmont J, Sullivan JS, Roche M, Zaunders JJ, Gabuzda ...Resources on Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral...

... specificity of monoclonal antibodies, they also lead to more reproducible experimental results. When ...NBK100203/ Additional resource on the Addgene blog Learn more about research applications for antibodies ...Studies Hybridoma Bank, a repository for hybridomas  Learn about monoclonal antibodies for immunotherapy Resource...

...receptor-transcription factor fusion. TEV protease cleave the transcription factor from the receptor, which... in chemogenetics  While there’s still a lot to learn about GPCR signaling, researchers in the meantime...02775-8 Additional resources on the Addgene blog Learn more about TRUPATH Read our quick guide to chemogenetics...

...forward-looking. Embrace it, and let your personality leap out of the page. Finally, as previously mentioned...the recommender knows you the better, and their leadership status in their current field of expertise augments...But you create a network of friends, and future colleagues that can help pull one another up while you are...

...’s wasting everybody’s time if you don’t. Add a clear cover letter to your submission, briefly summarizing...house style and prerequisites of the journal. Ask colleagues (preferably native English speakers) to proofread...that all required information is provided. The clearer you write your paper and the more information you...

...targets. Check out our post on Biotinylation to learn more about biotin and its use in molecular biology...other classes that we would be remiss not to at least mention. Oligonucleotides allow for sensitive detection... with BioRender.com.     Many biologists can lead perfectly blissful, productive lives without thinking...

...proteins in situ. I was privileged to be invited to learn from them at the MPI in Frankfurt am Main and perform...concerts and bake. you can contact her at rojas@ti2learning.org. References 1. Dieterich, Daniela C., et al...Fluorescent microscopy techniques - Which is best for me? Learn how small molecule fluorophores can be used for ...

...ever like to write about a recent plasmid deposit please sign up here.          Listen to this episode ...Acr activity as a biological tool, Niopek and colleagues turned to optogenetics. Through the use of light-sensitive...usually expressed from a different plasmid, which may lead to unequal gene dosage and high variability of protein...

...estradiol for hER or progesterone for hPR - leads to nuclear localization, binding to the appropriate promoter...ever like to write about a recent plasmid deposit please sign up here. No time to read? Listen to our Hot...

... works best for genome editing  with an active nuclease.  Read the paper in Molecular Cell Find the CasMINI...Programmed genome editing by a miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature...protect the RNA from degradation by cellular exonucleases and broadly improve editing efficiency. Read...

...people said it took them more than 22 hours, so at least the better part of four days. How much does it cost... a number of factors, but we can assume it’s at least equal to the $155 spent on reagents. Five ways to...tell. But even from these rough estimates, it’s clear that community engagement with and use of repositories...