...asking what approach will I take and what can I learn, learning the difference between good and bad data, and...establishes your professional brand. It offers colleagues insight into your interests and will determine...

...with lentiviral preps for the delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators, Cas9 repressors...expertise you’d like to contribute to the blog, please email us and we’ll get you writing!     www.addgene.org... Service or any of the materials we distribute, please send an email to [email protected]. Your questions...

...been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array of topics pertinent...Plasmids 101 2nd ed, the 3rd edition has now been released), and have started providing recorded interviews...Resources at Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository ...

...genome engineering methods for C. elegans were released, signaling a new era in C. elegans research. Homology...on the first attempt for 6/7 genes. The data are clear - the SEC-based system is both simple to use and...rapid, and user-friendly system represents another leap forward for the CRISPR/Cas9 revolution!   References...

...hope that this provides a clear overview of what you need to submit data. Please note that the data hub ...generated with a caption. For microscopy images, please include channel imaged, a color legend for probes...

...viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our...that govern such activities as neurotransmitter release is vital in studying neural networks. We have expanded...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...

...suggesting that outcomes are already determined by at least day 13. However, distinct transcriptional states...335547. Additional resources on the Addgene blog Learn more about induced pluripotent stems cells (iPSCS...delivery of reprogramming factors for generating iPSCs Learn how fluorescent proteins help researchers study ...

...antibodies are supplied with no sequence information, leading to problems with reproducibility and an inability...to polyclonal or monoclonal antibodies. You can learn more about recombinant antibodies in this blog post...Addgene blog Check out our Antibodies 101 blog post Learn more about plasmid-based recombinant monoclonal ...

...ever like to write about a recent plasmid deposit please sign up here. Listen to the Hot Plasmids Podcast...technologies based on polymerase processivity. Please find the MarathonRT plasmid here! Zhao C, et al...used to target any protein fused with GFP, YFP, mCerulean, or other related fluorophores. Find all of the...

... nearing deadline (an animated video depicting nuclear fission; a podcast with a genetics researcher on...condensed into two discernible paths. The first leads into the world of industry. Along this road, science...Understanding just how the public utilizes insights gleaned from scientific research is, after all, one of ...

... A diagnostic digest is one of the easiest and clearest ways to determine if your sample has recombined...extraction to isolate the full, intact plasmid. Please see our gel extraction protocol for more details...more molecular biology and viral vector protocols Learn about the common lab E. coli strains Find tips for...

...move, such as T lymphocytes. Griesbeck and his colleagues began by isolating a calcium-binding protein,... also points out that the approach he and his colleagues took to optimize sensitivities can now be applied...calcium, requiring greater sensitivity. He and his colleagues came up with a solution: they devised self-associating...

...depends on things being organized, tracked, and clearly communicated. And that’s where MTAs, and similar...how the shared reagents should be used. Setting clear expectations from the beginning makes the sharing...sharing process smoother, more consistent, and more pleasant for everyone, which in turn encourages people ...

...ever like to write about a recent plasmid deposit please sign up here.        New toolkit for N or C terminal...widely used genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for a specific...and follow the DNA sequence targeted by the Cas9 nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires...

...for reproducibility because mistakes in this can lead to wasted time or retractions. Using a catalog number...Bauer (Addgene plasmid # 87155 RRID:Addgene_87155)” Please note that we only provide recommendations for how...Check out this post on Scientific Reproducibility Learn about the anatomy of a plasmid page at Addgene ...

...dysregulation. A targeted TF screening approach can not only lead to a better understanding of disease onset, but ...isoforms. Due to the size of the collection, we’re releasing individual plasmids in batches. If there’s something....2017.016. Additional Resources on the Addgene Blog Learn More about Lentivirus Figure out How to Get the ...

...results as soon as the gels ran.” Dr. Ellis tries to lead his team as a scientists and a friend. George Church...3 for tips on how to develop your role as a lab leader, and then on to Chapter 4 for advice on staffing... labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early Career Scientists...

...search tool updates. Now, let’s take a look at the least visible part behind these changes: the backend technology...engine! Additional resources from the Addgene blog Learn about other features on Addgene's website Find a...step-by-step guide to using Addgene's new search Read to learn more about why we redesigned our homepage and mascot...

... specificity of monoclonal antibodies, they also lead to more reproducible experimental results. When ...NBK100203/ Additional resource on the Addgene blog Learn more about research applications for antibodies ...Studies Hybridoma Bank, a repository for hybridomas  Learn about monoclonal antibodies for immunotherapy Resource...

...receptor-transcription factor fusion. TEV protease cleave the transcription factor from the receptor, which... in chemogenetics  While there’s still a lot to learn about GPCR signaling, researchers in the meantime...02775-8 Additional resources on the Addgene blog Learn more about TRUPATH Read our quick guide to chemogenetics...