...scientists standing on the shoulders of their colleagues and all those who’ve gone before them. Given ..."The Secret of Life." 10)     And, last but not least (certainly not for us), Addgene launched this blog... Year, let's make a toast to open science. And, please, share your favorite OA developments of the past...

...openness and trust that comes from caring about your colleagues on a personal level, and employees often cite...project. These projects could be anything from cleaning out the freezers to writing new plasmid protocols... it? Perhaps new ideas or collaborations, or at least a shared sense that we’re not alone in struggling...

...planning, it shouldn’t be used in isolation. Without clear dialogue between trainee and PI, it’s easy to miss...Starting with a recap of accomplishments, from learning new techniques to finally figuring out a tough...the yearly planning meeting is that the trainee leads the conversation. As the conversation proceeds through...

...and easily. These tagged plasmids also include cleavage sites should one need to remove the tag post-purification...on these and other tags used in the pCri system, please see Plasmids 101: Protein Tags. Beyond E. coli...purification. For a more thorough introduction to pCri, please see the original publication cited below. pCri ...

... vs. SpCas9, see the table below and read on to learn more about GeoCas9.   SpCas9 GeoCas9 Size 1368...experiments tested the ability of GeoCas9 RNPs to cleave substrate when the reaction was carried out at ... in up to 30% plasma. The discovery of GeoCas9 clears the way for CRISPR/Cas9 genome editing in facultative...

...some Addgenies. As the gala came to an end, it was clear that the Addgenies had no intention of ending the...tough but memorable one. At the gala, one thing was clear: the sense of family is still the backbone [ba-dum-chh...helped to edit and add to this post. Interested in learning more about the last 10 years @Addgene Check out...

...nucleus in space and time, and to understanding how nuclear organization varies across the life of a cell, ...dCas9 gets a colorful makeover Even without its nuclease activity, catalytically dead Cas9 (dCas9) has ...under lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the...

... repressors of gene expression in yeast Megan McClean’s lab generated an optogenetic toolkit that controls...repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders the NLS...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

...blog posts Learn more about retrograde AAVs Resources on Addgene.org Read our AAV guide Learn more about...Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience...

...method that uses iterative staining and chemical bleaching to allow for high-resolution imaging without degrading...the IBEX Knowledge-Base is named on the Zenodo release in keeping with their value of shared ownership...IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues...

...universities and Technology Transfer Offices were, at least, temporarily closing, these groups were willing ... would like to make them available to industry, please reach out at [email protected] and we will...Addgene blog Browse blog posts related to COVID-19 Learn more about the Ginkgo COVID-19 plasmid collection...

...those collaborations over the years, he and his colleagues built a Molecular Expressions collection including...much easier for scientists everywhere to get a clearer picture of that intracellular world, even as the...promote the great work of Michael Davidson and his colleagues." Acknowledgments Thank you to Michael Davidson...

...It’s clear that CRISPR-Cas9 technology has really changed the game for anyone looking to quickly and ... positive selection screening. They and their colleagues including Feng Zhang used the GeCKO library to...CRISPR libraries developed by MIT's Tim Wang and colleagues, including David Sabatini and Eric Lander, comprise...

...require the use of specific sequences. Read on to learn how easy this process is and to get tips for applying...not well understood, it is believed that the exonuclease activity of the polymerase generates sticky ends...fidelity DNA polymerases like phusion. An 18-cycle PCR leads to the most colonies after transformation. When ...

...There’s a steep learning curve for Inkscape, but there are lots of resources for learning the software. ...media for scientists Find science career blog posts Learn about other lab software ...

...cells expressing Fcγ receptors binds to IgG and release cytotoxins that kill the target cell. If the pathogen...antibodies and eosinophils, where the eosinophils release cytotoxins via degranulation.  Antibody-dependent... as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies...

...Product Development Manager at eScience Labs. To learn how Caitlin got into scicomm and landed her first...series! Additional Resources on the Addgene Blog Learn more about alternative science careers here Interested... for scientists here Resources on Addgene.org Learn more about careers at Addgene ...

... anti-phage CRISPR effect observed in bacteria. Learn more about the different CRISPR applications in ...23256573. Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant... CRISPR for plants Bacterial Expression Systems Learn about Global Plasmid Distribution ...

...scientific data depends on validated reagents along with clear documentation, so we were excited when Amos Bairoch... cited. If the information seems incomplete or unclear, Bairoch follow up via email to the authors of ... you’re excited about this resource and want to learn more, check out the Cellosaurus FAQ! References...

...CRISPRi). A) Lentiviral vector design. B) Strategy for leak-free transcriptional control. C) Iterative transduction...activation in plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures up to 43...The guides are optimized to maximize on-target cleavage and minimize off-target effects.   New from ...