... proximity of the fluorophore and subsequently leading to a positive signal. The same effect can be achieved...this approach less sensitive in most applications. Learn About Fluorescent Biosensors Light-up aptamers (...assay. In 1999, Grate and Wilson paired laser cleavage and an RNA aptamer that bound the compound malachite...Addgene Blog Tips for Using FRET in Your Experiments Learn more about GFP Controlling Protein Activity with...

...A little over a decade back when Yamanaka and colleagues reported that it is possible to reprogram differentiated...fibroblasts and keratinocytes to peripheral blood mononuclear cells (PBMCs). PBMCs isolated from whole blood...Episomal vectors are based on the Epstein-Barr Nuclear Antigen-1 (oriP-EBNA-1) system where each vector... peripheral blood mononuclear cells...

...and ssDNA cleavage by canonical base excision repair enzymes. At the same time Cas9n cleaves the opposing...editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/...and enrichment of DNA editing by APOBEC–Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Research...

... around, the plasmids are randomly positioned, leading to plasmid loss (Diaz et al., 2015).  Figure...designed small, high copy incompatible plasmids that lead to asymmetrical plasmid loss of the usually large...Addgene blog Read all of our Plasmids 101 blog posts Learn more about the origin of replication Find blog posts... Leah Schwiesow...

...Cas13, the "collateral" activity of the enzyme can lead to off targets and result in significant, negative...processing pre-CRISPR RNA into mature CRISPR RNA and cleaving RNA at positions defined by the target:spacer ...limited to small deletions and small insertions, leaving a several kilobase-sized gap in the gene editing...Abudayyeh. Drag-and-drop genome insertion without DNA cleavage with CRISPR-directed integrases bioRxiv 2021. ...

...researchers recognize their MANY transferable skills learned while working in the lab and offering advice on...think of creativity as a form of innovation, it’s clear that creativity is absolutely a transferable skill...get distracted by an email or sidetracked by a colleague. To innovate, you’ll have to focus. Find yourself...(2019). Embrace your weird face your fears and unleash creativity. Gallery Books. Boyes, A. (2019, November...

... Make it clear that you are interested in the specific position you are applying to. Be clean and professional...letter, and if possible, send it to a mentor or colleague for a second look and helpful feedback!  What ...     Additional Resources on the Addgene Blog Learn to Develop Your Professional Network Become A Good...

... Flow data is messy, so learning how to gate takes a lot longer than learning how to read a gating strategy...visualization tool, a quadrant will often be too clean for real-world data. Most plots will have a shape...    Of course, there are many more things to learn about when diving into flow cytometry data. There...

...also reversible: once the environmental trigger leaves, gene expression is no longer activated. However...N2A, 3T3) and organisms (Chavez et al., 2016). To learn more about the different types of Cas9 activators....3149 Additional Resources on the Addgene Blog Learn how to Choose the Best Cas9 Variant for Your Next...More about Genome-wide Screens with CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on ...

...elements, insulators, and silencers; however, some "leakiness" of transcription may occur. This is normally ...small RNA expression shRNA From the human U6 small nuclear promoter Constitutive  Murine U6 is also used, ...lacI or lacIq). Can be induced by IPTG or lactose. Leaky promoter with somewhat weak expression. lacIq mutation...the Addgene Blog Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter Gene...

...reproducibility. These alarming results are due, at least in part, to insufficient and non-standard record...exchange information regarding lab work with your colleagues, supervisors, clients or even students, your ...track and find data and information. If an employee leaves the institution, the transition is smooth as all...scinoteELN Additional Resources on the Addgene Blog Learn about more tools for reproducibility 5 great apps...

...degraded by uracil DNA glycosylase (UDG) and Exonuclease III (ExoIII) (step 4). UDG recognizes uracils...from newly synthesized DNA. Lack of both enzymes leads to a buildup of uracil and the insertion of uracil...limitation is sequencing depth and cost. You need at least 100-200 reads per variant, so the more codons you...to Generate Specific Mutations in Your Plasmids Learn How You Can Use CRISPR Pooled Libraries to Generate...

... the nonhomologous end-joining (NHEJ) pathway, leading to nonspecific nucleotide insertions, dele­tions...allow blocking the PAM sequence (e.g. if it would lead to a missense or nonsense mutation), blocking the...the PAM as possible (see below to find out why). Please check our recent studies for details (Paquet, Kwart...28102837. Additional Resources on the Addgene Blog Learn about High Fidelity Cas9 Variants How to Design ...

...protein is cleaved by proteases. Nowadays, with ample structural information available, it’s clear that the...protein is mislocalized. The APT1-mVenus fusion is clearly a better choice to work with. Inserting a fluorescent...the existing pool of protein within a cell and may lead to over-expression artifacts. This is an important...

...Addgene blog Learn about research applications of antibodies in Introduction to Antibodies Learn more about...visualization methods. Stay tuned for a future blog post to learn more about these techniques!  Direct vs. indirect...what sort of protocol you will need to use. (To learn more about antibody validation, this commentary ...

...Optogenetics Guide to learn more about how these modules work, and read on to learn how they are put to ...factors (GEFs) activate their partner Rho GTPase, leading to behaviors like lamellipodia or filopodia formation...these physical processes. Joachim Goedhart and colleagues in the Gadella Lab devised a set of optogenetic...

...problems. This can lead to a lack of trust in the mentor and this training is clearly not equitable to ...assess the current state of research became more clear throughout the various sessions of the symposium...first couple of sessions of the symposium it was clear that, even though postdoc associations across different...

...strain-specific tropism.” For example, AAV-PHP.B doesn’t lead to enhanced transgene expression, compared to AAV9...mice use LY6A to cross the blood-brain barrier, leading to strain-specific tropism (See S3 table (Huang... can visit our Caltech Systemic Capsids page to learn more about that collection. Table 1: Systemic capsid...systemic AAV experiments and Addgene’s AAV Data Hub to learn more about the capsid you’re interested in. Then...Fish, K. N., & Pfenning, A. R. (2022). Machine learning sequence prioritization for cell type-specific...Troiano, E. C., Moghadaszadeh, B., Peacker, B. L., Leacock, K. A., Horwitz, N., Beggs, A. H., Wagers, A. J...

...expression, but causality and mechanisms remain unclear. Today, targeted epigenetic modification at specific...came epigenome-engineering tools - zinc finger nucleases and TALENs fused to epigenetic modifiers enabled...Christopher Newgard’s Lab has fused LSD1 to the smaller nuclease-dead Staphylococcus aureus Cas9 (Sa-Cas) and deposited...comments! Find Plasmids for Epigenetic Modification   Leah Schwiesow contributed to updating this article. ...

...multimer present in your sample. In these instances, please note that the supercoiled dimer band can sometimes... presence of a multimer in your sample remains unclear after visualizing it on a gel, one of the other...consistent with the size of a supercoiled plasmid dimer. Please note that it is common to see other plasmid confirmations...Ausubel FM. Recombination between bacterial plasmids leading to the formation of plasmid multimers. Cell. 1976...