Components of CRISPR/Cas9
Type
Blog Post
Published
Feb. 2, 2016, 5 p.m.
...have two endonuclease domains: the n-terminal RuvC-like nuclease domain and the HNH-like nuclease domain...conformational change that positions the nuclease domains to cleave opposite strands of the target DNA. Thus...and Cas9, bind to these transcripts and C) cleave them leaving mature crRNAs bound to the Cas9/tracrRNA ...Cas9 complex to the target DNA which is E) cleaved leaving a F) double-strand break. A “gRNA” is a researcher-designed...Cas9 genome editing systems use a non-specific endonuclease (Cas9 or closely related Cpf1) to cut the genome...genome and a small RNA (gRNA) to guide this nuclease to a user-defined cut site. After reading this post...functions of the various CRISPR/Cas9 components. Please note that while this post is intended to provide...