...even to not bind to their target molecule at all, leading to inconclusive results, erroneous conclusions,... that your antibody has been validated using at least one of these strategies. Choosing which strategy...) the protein of interest in your sample, should lead to reduced or eliminated antibody signal. If you...translation start sites or alternative splicing that could lead to some form of the target protein to be present... Check out our Selecting an Antibody blog post! Learn more about Antibodies and their many applications...

...alcohol-based disinfectants. A 10% bleach solution should be used to clean AAVs from workspaces. Speed...Manitoba due to their Biosafety Level-2 or 2+. Please Note: Despite the fact that AAVs themselves are...vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune response. Any investigator who ...antisense oligonucleotides (ASOs), zinc finger nucleases, and many others (12). The most attractive potential...

...countries. We, like everyone around the world, are learning to navigate the changes brought about by the pandemic...opportunities. I have spent the last 9 months quietly learning about Addgene’s past and present, but the real...successes to reflect upon, a wealth of history to learn from, and a compelling future to plan in the changing...people. I look forward to working with Addgene colleagues, collaborators, partners, and the larger scientific...

... change? Addgene has you covered. Today we’re releasing 11 bold science-themed backgrounds! Show off your...out Addgene’s Fluorescent Proteins blog topic to learn more about FRET and other techniques using FPs! ...Addgene are shipped as bacterial stabs? You can learn about what format your material will come as (and...molecular biology tools in your research or not! And learn more about the variety of careers available to scientists...

...applications was still very new. Now, Konermann and her colleagues including Zhang and Mark Brigham have taken the...influences in our environments. We are able to react and learn. All of this requires genes to be regulated dynamically...modifications in the genome,” Zhang said in MIT’s press release. In an accompanying Nature News & Views Andreas...processes and disease states. While LITE plasmids have clear application to anyone with an interest in neuroscience...

...plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though ...with the plasmid post transfection. The 2A "self cleaving" peptide causes the ribosome to translate Cas9...gRNA that will target SpCas9 to the approrpriate cleavage site). Finally, in addition to the 2A peptide ...Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some...

... guide Learn about different careers in science communication Resources on Addgene.org Learn how Addgene...main three points of your story. Interested in learning more? Visit their website at www.theaddictivebrain.com.... Sadangi is an eLife ambassador, SfN community leader, and an early research career panelist at BioTechniques...

...Resources at Harvard Medical School, Joanna was my colleague. She mentioned Addgene was looking for someone...jewelry making, reading, sailing, and gardening. Learn more about Julie Cicalese  Ms. Cicalese served as... for Harvard Medical School where she brought leadership teams together to advance the mission of the ...England. Additional resources on the Addgene blog Learn more about about Addgene in our "Inside Addgene"...

...King soundtrack and writing this post, it is my pleasure to announce that we once again reached new heights...section to make this post even more useful to your colleagues. Technique: Probe Phage Genomes for Host Binding...(check out our interview with Michael Koeris to learn how his company uses phage to test for Listeria ...posts in chronological order here: Four Things I Learned at the 10 Years of iPSCs Symposium Tips for Writing...

... well-integrated into our lab lives. Read on to learn about some creative approaches to lab sustainability...Who even regulates them? You may be surprised to learn that it hasn’t even been 50 years since the creation...fluidic machines handle biological samples and are cleaned between each use. You wouldn’t throw away your ...series!   Additional resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe...

... passion, and patience are required to build and lead a productive and successful research lab. Once goals...champagne, that's a given.” Furthermore, anyone leaving the lab receives send-off via a gift, card, and... the afternoon during the child-rearing years, learning “lots of interesting things from children, their...their classmates, and parents”. He also cooks and cleans with his family, “sometimes listening to audiobooks...

...functionality can be found in the following video: Please remember that the accuracy and reliability of our... plasmid is missing from our collections pages, please shoot us an e-mail at [email protected]. We’d love...visibility in a few other ways. Every quarter we release a Newsletter where we spotlight some of the plasmids... in the repository. And last, but certainly not least, we often feature new plasmid technologies on our...

... also have the option to “Leave us a message.” When you click on the "Leave us a message" button shown...lab’s materials and requesting them from your colleagues, to intellectual property rights and handling...the Addgene Blog Read other Inside Addgene Posts Learn about New Features on Our Plasmid Pages Resources...

... year. The society facilitates opportunities to learn about Synthetic Biology and exciting new technologies...network of fellow scientists that you can grow with, learn from, and mentor while pursuing your research interests... which would benefit from some Addgene support? Please get in touch, as we aim to support a couple of ...Networking Get Career Advice from Addgene Bloggers Learn How to Get Involved in a Profession Organization...

...more years with a small number of formal advisors learning how to be a scientist. It is shocking how little...important in both cases. As a PhD student you need to learn how to be a scientist. Your PI should be the kind...science career. Will you be given opportunities to learn and practice successful grant writing, lab budgeting...Harvard Medical School researcher Connie Cepko to learn about her mentoring style. How can I find out if...

...genome. Cas genes, which code for polymerases, nucleases, and helicases, were also disrupted to elucidate...CRISPR loci to generate RNA, which guide a Cas nuclease to the specified exogenous genetic material next...within a host cell. A few virus particles have learned to circumvent the CRISPR/Cas defense by generating...mutation in the PAM sequence, preventing the Cas nucleases from re-identifying it (3). Surprisingly, few ...

...hyperthermostable Cas9 This year it was wonderful to learn that one project led by postdoc Dr. Stephanie Schmidt... IgnaviCas9 is a hyperthermostable Cas9 CRISPR nuclease that is active up to 100°C.  The IgnaviCas9 protein...for long time periods and workflows where CRISPR nuclease activity is desired and time or protocol steps...useful. Additional resources on the Addgene blog Learn about other Cas proteins Read all of our CRISPR ...

... you want. But how do you know if a masters will lead to a career you will like? Here’s two things to ...setting up an informational interview with them to learn how their master's degree prepared them for their... help with manuscript and grant preparation, and lead your own research project.  3. Do you prefer being...eBook.   Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now...

...insights discoverable. Watch this quick video to learn how ReFigure works and continue reading for more... and again as they don’t save the insights they glean from a reading session. To remember what happened...reach the ReFigure team at [email protected] Please support ReFigure by creating ReFigures and answering...video to share your science at We Share Science Learn more about Data Sharing Resources at Addgene.org...

...tertiary structure when transcribed which aids in nuclear export of mRNA and helps increase the expression...The polyadenylation or polyA signal aids in the nuclear export of RNA and RNA translation, and promotes....2019.276 Additional resources on the Addgene blog Learn more about basic viral vector elements Read about...about four considerations for the perfect AAV tool Learn about synthetic promoter AAVs for cell-type expression...