...effect reprogramming rates and such a number maybe misleading. Integrating iPSC delivery methods Overall, integrating...incomplete silencing of reprogramming transgenes, which leads to incomplete reprogramming. Additionally, lingering...suppressor genes and/or oncogenes, potentially leading to the presentation of a cancer-like phenotype ...regulating transgene expression. Check out Sano et al to learn how microRNAs can be used to regulate Sendai virus... don’t integrate or replicate. Their small size leads to higher transfection efficiencies and they tend...completely removed.  Replicating oriP/Epstein-Barr nuclear antigen-1 (EBNA1) based episomes: These plasmids...all applications of iPSCs, but there should be at least one method that can help you with your research ...

...team costumes, and spooky science stories. Yes, please! At Addgene, we love finding ways to incorporate...their behavior. These ants become “zombies” and leave their foraging trail. They climb upwards until they... The ants bites down and locks their jaws into a leaf, and dies as its muscles atrophy. After the ant’...ant’s body. The fruiting bodies then rupture and release spores onto the forest below for the next unsuspecting...adult worms develop in the digestive tract and release eggs in the bird feces, which get eaten by another...water. There, the worm emerges to find a mate, leaving the insect dead or dying in the water. It’s thought...

...targeting integrins, will be released early this year.  In September, we released the fourth edition of our...the recent release of the Spanish version of our CRISPR 101 eBook and the upcoming release of the second...services available in South Korea.  In addition, we released the MORF collection, a comprehensive transcription... our export license application service, we’re pleased to announce that we are now able to ship rabies...

...their research leading up to the discovery of the mechanism of dual-RNA-guided DNA cleavage in adaptive ...crRNA could be combined into one RNA to direct cleavage the result was a simpler 1 protein - 1 RNA system...cells but this can’t be used in people until we learn more about safety and more about the how to use ...cool biology can be and how much we have yet to learn. As Dr. Mello said, “Cells have been in the information...sophisticated”.     New to CRISPR/Cas9 Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR...

...England Biolabs and Labconscious. I was eager to learn about practices I could bring both to Addgene, and...impact as scientists. Many scientists are already leading the cultural change toward responsibility in our...polystyrene will likely end up in a landfill, but at least we can reduce the rate at which that happens and... it offsets the cost of regular waste services. Learn about how to start a glove recycling program at ...on culture. Encourage your workplace to become a leader in sustainability. Sign up for blog posts and...companies, and universities to be sustainability leaders. Take initiative to bring ideas to your workplace...

... sites in a promoter lead to higher transcriptional activation. Multiple nuclear localization signals ...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted... ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking...SunTag plasmids have coveted “blue flames,” it’s clear that the system is broadly applicable across many...eBook Avoid Fluorescent Protein Oligomerization Learn about Small Molecule Fluorphores Resources on Addgene.org...

...exploration. Here, we delve into the importance of leaving room for serendipity during your PhD studies and...often happen when we allow ourselves moments of release and mental freedom. Practical approaches Engaging...not just a stroke of luck; it's a mindset that can lead to unexpected discoveries and innovative solutions...discoveries. So, on your academic journey, remember to leave a little space for the unexpected — it might just...Academia: Stories and Strategies for PhDs Making the Leap to Industry." He successfully transitioned to industry...Experimenting with New Careers While in Grad School How to Lead a Great Meeting Form Your Own Peer Mentoring Group...

...serve them better. Here’s a little bit of what we learned. Check Out Our Microbiology Page! The start of ...improving lives worldwide and the presentation made it clear how important it is for us to improve our resources...science education and outreach ourselves, we can a learn a lot from these ASM pros and hope to leverage their...was also a great place to catch up with former colleagues! I ran into past ASM President Tim Donohue (see...their materials! Resources on the Addgene Blog Learn How You Can Use Addgene's Microbiology Pages Read...CRISPR Methods for Bacterial Genome Engineering Learn how to Network at Scientific Conferences Resources...

...Flame Awards. What's in a flame?     We’re always pleased when scientists around the world choose to share... serves to congratulate researchers who have at least one plasmid deposited at Addgene that has been distributed...Sainsbury Laboratory in Norwich, UK: “We’re very pleased to distribute our plasmids via Addgene. Given the... us hours of work responding to requests from colleagues.” In addition to helping labs save time, we’re... plasmids and have not yet received your award, please contact [email protected] to claim your Blue Flame...deposit. Begin yours here. It has been my genuine pleasure to send these small tokens of thanks for the incredible...

...Loren Looger’s lab, Kaspar Podgorski and colleagues have now released iGluSnFR3 (Aggarwal et al. 2022). This... oxytocin, Daisuke Ino and Hiroshi Hibino and colleagues devised a “Modular fluorescence unit fused with...voltage sensor The François St-Pierre lab and colleagues have developed a new genetically-encoded voltage...limited. In a new preprint, Jared Toettcher and colleagues describe pYtags, a modular system for monitoring...dynamic range. With this in mind, Ed Boyden and colleagues set out to engineer a potassium ion biosensor... signal/noise ratio. Here, Takeharu Nagai and colleagues present an improved genetically encoded temperature...

...basis of the Surveyor assay is that Surveyor nuclease cleaves double stranded DNA (dsDNA) at basepair mismatches...mismatches at the site of the edit. Using Surveyor nuclease followed by gel electrophoresis, these mismatches...mismatches are indicated by the presence of DNA cleavage products. The appearance of the 428 bp band indicates...I designed (listed above) worked well and I got clear bands using the conditions above. 2. Design your...product, you would still be able to identify the cleavage product. 3. The Surveyor kit had a lot of good...Before digesting the DNA duplexes with Surveyor nuclease, save some of the duplex and run it as an undigested...

...of scientists from MIT tapped into this data to learn about trends in synthetic biology and DNA synthesis...database. Through a series of simulations and machine learning, they created a classifier that predicts whether...gene and a synthetic version of that gene was a clear predictor of whether the gene is synthetic or natural...applications in monitoring the accidental or intentional release of engineered organisms. “Is [the organism] engineered...Nielsen, Alec AK, and Christopher A. Voigt. "Deep learning to predict the lab-of-origin of engineered DNA...Addgene blog Read blog posts about synthetic biology Learn more about codon usage biases Resources on Addgene.org...

...out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring group We don’t all...matter why, now is a great time to find a few colleagues to work together on career and life goals. For...scientist for show wrestling, founded a nonprofit, learned to play the ukelele...perhaps you get the idea....hope you all know by now that people get jobs at least 85% of the time because of relationships. It’s much...conversations. If you set yourself a goal of doing at least two informational interviews a month you will make...academia and not in academia is run by grant funding. Learning to write grants quickly, efficiently and effectively...

...of your colleagues. The young scientists that are reading your peer-review comments will learn from them...delivering constructive critique can help your colleagues at the receiving end, if not for this specific...repositories will ensure that the results of your colleague’s hard work will be maintained and made available...comment differently if I would be speaking to a colleague in the office next door. So be kind, helpful, ...writing a review article Resources on Addgene.org Learn about depositing plasmids pre-publication Find molecular...

...industry.” In his article On Leaving Academe, Terran Lane is asked by his colleagues and friends why he would...in the Boston area that demonstrate that 85% of leaving postdocs pursue a career outside the traditional... like this without sufficient data? Starting to learn about the diverse options and how to take advantage...there. Additional Resources on the Addgene Blog Learn about Possible Science Careers How to Negotiate ...for Scientists Series Resources at Addgene.org Learn About Careers at Addgene ...

...on Precision Genome Editing with Programmable Nucleases. Check out #KSgenome on Twitter if you missed ...the CRISPR field forward. To do that, we need to learn much more about cellular DNA repair pathways. An...An emphasis on cellular repair post-CRISPR cleavage When I first looked at the conference agenda, I was...would open not with an introduction to CRISPR nucleases, but with a session on DNA repair. What stuck ...) Delivery The RNP delivery method consistently leads to high on-target editing and low off-target editing... tool supports over 250 genomes, and they just released a brand new CRISPOR manual to kick off 2018! "...

...start – it is the discussion after that will lead to learning. Listen to our podcast interview with Harvard... take informal minutes on what was covered and learned, especially noting action items that are identified...Harvard Medical School researcher Connie Cepko to learn about her mentoring style. Set goals with a mentor.... There are many advantages to being a Mentor: Learn other perspectives Develop coaching skills Encourage...Encourage your creative thinking Enhance leadership skills Support inclusion, diversity, and open communication...

...while the alcohol used in the staining process bleached another, Klebsiella pneumoniae. After realizing...safranin, as a counterstain to help visualize the bleached bacteria. The chemistry behind Gram’s crystal ...drop by drop onto the smear until the alcohol runs clear and immediately rinse. Flood the smear with the ...Rinse the slide in tap water until the runoff is clear, blot gently to dry, and then view under an oil ...peptidoglycan membrane is unable to retain the stain, leading to its decolorization. In contrast, counterstain...visualizing differentiations between bacteria, leading to safer medicine and more successful experiments...

...drug discovery, and synthetic biology. Read on to learn more about the five awardees and their promising...their use as food, infusions of L. taraxacifolia leaves are widely used to treat several diseases. Her ..., the most common type of stroke and the third leading cause of death and disability worldwide. An ischemic...ischemic stroke occurs when arteries leading to the brain become blocked, resulting in reduced blood flow...awards. Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant...Expression Systems Synthetic Biology Resources Learn about Global Plasmid Distribution ...

...game that would force my students to use concepts learned from previous lectures, articles and books. A game...board with them. My impression was that they had clearer ideas about gene therapy in the following lessons...perhaps the ideas spurred by this “gamification” will lead to new cures for disease someday! Accessing and ...modify BUILD YOUR PLASMID or generate games like it, please freely share your projects with us! This exchange...want to receive our modifiable powerpoint file, please feel free to contact us at: [email protected] tools for teaching developmental biology Learn how BioBuilder can help teach students about Synthetic...