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Showing: 61 - 80 of 991 results

  1. Viral Vectors

    Type
    Collection
    ...Which Type of Virus Should I Use? Use this guide to learn more about the different types of viruses that are...select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit ...viruses are temporary in the host. Use this guide to learn more about the different types of viruses that are...depending on the insert type and envelope used. Please consult with your institution’s Biosafety Committee...

  2. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Deisseroth AV-1-PV3248 105557-AAV1 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG James M. Wilson AV-1-PV3508 98928-AAV1 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...Wickersham AV-9-PV3248 105557-AAV9 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG James M. Wilson Cre Recombinase AV-1-PV1090...Plasmids are available for all the items listed below. Please let us know (Link opens in a new window) what you...you would like to see available in viral format. Please note this does not guarantee viral service, but...pENN.AAV.CB7.CI.TurboRFP.WPRE.RBG James M. Wilson Please contact [email protected] with any questions. Return...

  3. TALEN Plasmids and Kits

    Type
    Collection
    ...pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity ...DNA-binding domain of a TALE, along with strong nuclear localisation tags and repress transcription initiation... BioSciences (N153AA, C63AA) and showed robust cleavage activity in several later studies. pCAG-T7-TALEN...

  4. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...- Insect expression vector from Gileadi lab Xenopus SP6 for transcription and injection...full Fluorescent Protein Guide NLS Nuclear Localization pENTR4-myc-nuc - pEntry ... vector that adds a C-terminal nuclear localization signal FNpcDNA3 - N-terminal ...N-terminal FLAG and SV40 nuclear localization signal Myr Membrane Localization pWZL-Neo-Myr-Flag-DEST...

  5. Calarco Lab CRISPR Plasmids

    Type
    Collection
    ...12targeting sgRNA. Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase...publication. For questions regarding the protocol please contact Dr. John Calarco: [email protected]...

  6. Doudna Lab CRISPR Plasmids

    Type
    Collection
    ... Design Tools CRISPR Blog Posts Assorted Cas9 nucleases from different bacteria as well as a mammalian...described in: A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity. Jinek M, Chylinski...structure-specific RNA processing by a CRISPR endonuclease. Haurwitz RE, Jinek M, Wiedenheft B, Zhou K,...

  7. Kamoun Lab CRISPR Plasmids

    Type
    Collection
    ...work in the model plant Nicotiana benthamiana . Please refer to the Supplementary methods section of the... Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nekrasov V, Staskawicz B, Weigel D, Jones JD...pK7WGF2::hCas9 : Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with ...

  8. Boxem Lab CRISPR Plasmids

    Type
    Collection
    ...hap-16.48 promoter. Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase...optimized for C. elegans expression, flanked by two nuclear localization signals. pMB62 and pMB63 drive expression...

  9. AAV Viral Preps

    Type
    Collection
    ...new window) (Penn Vector Core) and Addgene are pleased to announce a partnership to provide high-quality...Suggestions Don’t see what you are looking for? Please let us know what you would like to see available...filling out our Viral Service Suggestion Form . Please note this does not guarantee viral service, but...

  10. CRISPR Plasmids and Resources

    Type
    Collection
    ...Plasmids and Resources Addgene is working with the leading scientists in the field to assemble the reagents...links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial immune ...visualize genomic loci. dCas9-FokI dCas9 fused to FokI nuclease generates a DSB when expressed with two gRNAs....

  11. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...designed to be used with any CRISPR nuclease and function. Please see the publication or plasmid information...and maintained by the Doudna Lab . gRNA Plasmids Please note that the following table lists both published...

  12. Serotype Testing AAV

    Type
    Collection
    ...Looking For? Please let us know what you would like to see available in viral format. Please note this does... . Note: For a list of all 100 µL sized sample, please see our Control AAV Inventory . The catalog ID ...

  13. Biosensor AAV Preps

    Type
    Collection
    ...Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...Inventory Page . Don’t See What You’re Looking For? Please (Link opens in a new window) let us know what you...you would like to see available in viral format. Please note this does not guarantee viral service, but...

  14. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...information on ordering from a for-profit entity, please see our Plasmid Distribution to Industry page. ...you describe the use of a plasmid in an article, please fill out this Google Form and we will add your ...resources, such as reagents, data, news, and more, please see Ginkgo's Synthetic Biology (and beyond) Community...

  15. AAV for Neuronal Tracing

    Type
    Collection
    ...Looking For? Please let us know what you would like to see available in viral format. Please note this does...in trans . (3) Nascent G-protein coated RAVB is released from the starter cell and free to infect neighboring...

  16. Cre-lox system

    Type
    Collection
    ...Learn about the Cre-Lox system and how it can be used for genetic rearrangements and recombination. Use...Zeng 140135 pAAV-EF1a-iCreV Split Cre fused to nuclear localized wild-type VVD EF-1 alpha AAV Zeng 140284...expression of Cre Mouse Sncg AAV Hu 154018 LiOn-CMV∞Cre leak-proof iOn (LiOn) Cre switch CMV Mammalian Livet ...containing FLEx technology. Read the genOway press release for more information. Empty backbones to regulate...24334 hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean Heat-inducible reporter with Cre dependent H2B-RFP...

  17. Retrovirus Plasmids

    Type
    Collection
    ...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...enter the nucleus during mitotic breakdown of the nuclear envelope). Additionally, γ-retroviruses integrate...integrate randomly into the host's genome, which can lead to oncogenesis (termed insertional mutagenesis). ...

  18. Plant Plasmids and Resources

    Type
    Collection
    ...biology - deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for...Plant Base Editors Save Time with Transient Plant Leaf Transformations RNA Interference in Plant Biology...

  19. Zebrafish Plasmid Collection

    Type
    Collection
    ...site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make...Highlights of Zebrafish Tools in our Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR...

  20. Lentiviral Prep Service

    Type
    Collection
    ...Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate double-stranded breaks ( DSBs...Suggestions Don’t see what you are looking for? Please let us know what you would like to see available...filling out our Viral Service Suggestion Form . Please note this does not guarantee viral service, but...
Showing: 61 - 80 of 991 results