...reports have shown that engineered zinc finger nucleases (ZFNs) are effective tools for making targeted...the spacer sequence between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT...open-source' engineering of customized zinc-finger nucleases for highly efficient gene modification. Maeder...mutagenesis in zebrafish using customized zinc-finger nucleases. Foley JE et al. Nat Protoc. . 2009. Dec 3. 4(...

...Which Type of Virus Should I Use? Use this guide to learn more about the different types of viruses that are...select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit ...viruses are temporary in the host. Use this guide to learn more about the different types of viruses that are...depending on the insert type and envelope used. Please consult with your institution’s Biosafety Committee...

...Deisseroth AV-1-PV3248 105557-AAV1 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG James M. Wilson AV-1-PV3508 98928-AAV1 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...Wickersham AV-9-PV3248 105557-AAV9 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG James M. Wilson Cre Recombinase AV-1-PV1090...Plasmids are available for all the items listed below. Please let us know (Link opens in a new window) what you...you would like to see available in viral format. Please note this does not guarantee viral service, but...pENN.AAV.CB7.CI.TurboRFP.WPRE.RBG James M. Wilson Please contact [email protected] with any questions. Return...

...pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity ...DNA-binding domain of a TALE, along with strong nuclear localisation tags and repress transcription initiation... BioSciences (N153AA, C63AA) and showed robust cleavage activity in several later studies. pCAG-T7-TALEN...

...Learn more about chemogenetic resources available at Addgene. See our inventory of chemogenetic constructs...and how to use these tools in your experiments, please see our Chemogenetics Guide . Chemogenetic Plasmids...Mammalian expression, non-viral Viral Service Available Clear Filters ID Plasmid Receptor Promoter Reporter/Fusion...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...

...- Insect expression vector from Gileadi lab Xenopus SP6 for transcription and injection...full Fluorescent Protein Guide NLS Nuclear Localization pENTR4-myc-nuc - pEntry ... vector that adds a C-terminal nuclear localization signal FNpcDNA3 - N-terminal ...N-terminal FLAG and SV40 nuclear localization signal Myr Membrane Localization pWZL-Neo-Myr-Flag-DEST...

...modify a single genomic locus. Before ordering, please review the steps for using pooled CRISPR libraries...Genome-wide Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type Species PI Lentiviral...Weissman 3rd 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 Knockout ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...

...12targeting sgRNA. Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase...publication. For questions regarding the protocol please contact Dr. John Calarco: [email protected]...

... Design Tools CRISPR Blog Posts Assorted Cas9 nucleases from different bacteria as well as a mammalian...described in: A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity. Jinek M, Chylinski...structure-specific RNA processing by a CRISPR endonuclease. Haurwitz RE, Jinek M, Wiedenheft B, Zhou K,...

...work in the model plant Nicotiana benthamiana . Please refer to the Supplementary methods section of the... Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nekrasov V, Staskawicz B, Weigel D, Jones JD...pK7WGF2::hCas9 : Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with ...

...hap-16.48 promoter. Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase...optimized for C. elegans expression, flanked by two nuclear localization signals. pMB62 and pMB63 drive expression...

...Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...Inventory Page . Don’t See What You’re Looking For? Please (Link opens in a new window) let us know what you...you would like to see available in viral format. Please note this does not guarantee viral service, but...

...Plasmids and Resources Addgene is working with the leading scientists in the field to assemble the reagents...links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial immune ...visualize genomic loci. dCas9-FokI dCas9 fused to FokI nuclease generates a DSB when expressed with two gRNAs....

...designed to be used with any CRISPR nuclease and function. Please see the publication or plasmid information...and maintained by the Doudna Lab . gRNA Plasmids Please note that the following table lists both published...

...Looking For? Please let us know what you would like to see available in viral format. Please note this does... . Note: For a list of all 100 µL sized sample, please see our Control AAV Inventory . The catalog ID ...

...information on ordering from a for-profit entity, please see our Plasmid Distribution to Industry page. ...you describe the use of a plasmid in an article, please fill out this Google Form and we will add your ...resources, such as reagents, data, news, and more, please see Ginkgo's Synthetic Biology (and beyond) Community...

...Looking For? Please let us know what you would like to see available in viral format. Please note this does...in trans . (3) Nascent G-protein coated RAVB is released from the starter cell and free to infect neighboring...

...Learn about the Cre-Lox system and how it can be used for genetic rearrangements and recombination. Use...Zeng 140135 pAAV-EF1a-iCreV Split Cre fused to nuclear localized wild-type VVD EF-1 alpha AAV Zeng 140284...expression of Cre Mouse Sncg AAV Hu 154018 LiOn-CMV∞Cre leak-proof iOn (LiOn) Cre switch CMV Mammalian Livet ...containing FLEx technology. Read the genOway press release for more information. Empty backbones to regulate...24334 hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean Heat-inducible reporter with Cre dependent H2B-RFP...

...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...enter the nucleus during mitotic breakdown of the nuclear envelope). Additionally, γ-retroviruses integrate...integrate randomly into the host's genome, which can lead to oncogenesis (termed insertional mutagenesis). ...

..., additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can...or Cas9 domains PE7 - addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA...