... making targeted mutations in zebrafish. With support from the NIH and guidance from the academic community...

... in experiments such as immunoprecipitation and super-resolution microscopy. Two commonly used plasmid-based...

...Sponsor We are grateful to The Kavli Foundation for supporting this meeting. The goal of this year’s workshop...

... H1 Ataxia telangiectasia Didier Trono 14581 pSUPER.ATMi ATM H1 Ataxia telangiectasia Didier Trono 14666...RAB25 His T7 Parkinson's Cheryl Arrowsmith 26210 pSUPER TBK1 TBK1 H1 ALS Tom Maniatis 26365 pENTR4_FLAG... pDONR221_SLC1A3 SLC1A3 Episodic ataxia Giulio Superti-Furga 132389 AAVS1 CAG rtTA3 TauWT 2N4R-EGFP MAPT...pDONR221-SLC1A3_STOP SLC1A3 Episodic ataxia Giulio Superti-Furga 161582 pcDNA3.1(+)_Strep-Lrrk2-Myc LRRK2 ...

... cloning and expression systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors...

... cloning and expression systems) and therefore supplied as one item. Collections are plasmids grouped ...

...or a H1-shRNA cassette from another vector (e.g. pSUPER) can be cloned into this vector; Contains a 5'LTR...

...promoter’ in the tables for species-specific tools). Support open science in plant biology - deposit plant plasmids...

...linker mGFP-10-sREACh-N3 Monomeric EGFP attached to super-REACh via a 10 amino acid linker ACA Heterotrimeric...

...the following three buffers. All buffers are supplemented with 0.001% pluronic F68 or 0.014% Tween 20....

...Approximately 2 weeks post-thaw, cell culture supernatant is tested for mycoplasma contamination. To date...

...function. Browse plasmids or viral preps created with support from the BRAIN Armamentarium Project below. Plasmids...

...your experiments can be found below and in the supplemental materials from the Doyon Lab’s publication: ...

...compatible with truncated gRNAs to increase specificity SuperFi-Cas9 - increases fidelity with reduced nuclease...consecutive binding and degradation of negatively supercoiled invader DNA by cascade and CAS3. Molecular Cell...

...transmembrane glycoprotein of the immunoglobulin superfamily, binds to the SARS-CoV-2 S protein and is involved...

...opens in a new window) MMRRC Repository - NIH-supported mouse and cell line repository (Link opens in ...

... serotype will work in your mouse line, see Supplementary Table 3 (Link opens in a new window) . Browse...

...structure, separate chromosomes during mitosis, support transport of organelles and vesicles, and more....is not feasible or when the protein of interest supports a variety of processes in different cell types... (Aljiboury et al., 2023). Rab family proteins support vesicle trafficking and organelle dynamics, but...contexts are sure to reveal even more insights. Super-duper lipid editor And if recruiting proteins to...PLD). Now they present a set of variants called SuperPLDs with 10, 30, 50, and 100 times the activity of...other natural or synthetic head groups. The new SuperPLDs work with high efficiency in mammalian cells and...opening up a wide realm of possibilities. The CRY2-SuperPLD module is optogenetically recruited by different...

...the community as a whole could support Addgene as we have been supporting the community. While many were...Zuckerberg Initiative for supporting Addgene by providing a generous grant. This support will enable us to continue...We’re extremely proud of the work we’ve done to support COVID-19 research. Our collection of available ...operations for all ongoing processes are entirely self-supporting. We hire more staff as we need them to meet demand...approaching science funding organizations to request support for our operations until our normal work resumed...via Addgene. Perhaps if each of many grantors supporting research could provide Addgene with a small amount...Science, Cori Bargmann. "CZI is especially proud to support Addgene now, when their distribution of plasmids...

...handy if a supervisor wants to see the full activity on a project. Can you meet the ELN support team? We... is likely to require on-going maintenance and support.” Besides that, academic institutions tend to think...study also showed that It is important that an ELN supports all types of data, which enables researchers to...property across various software providers used to supplement their paper notebooks. Instead, all of the important...use the ELN i.e. do you need a system that will support your lab’s growth in the near future? As most payable...years, it is also good to check how the ELN will support that. In addition, if you are working for a large...information regarding lab work with your colleagues, supervisors, clients or even students, your ELN should allow...