...the pAdEasy-2 packaging plasmid; Supplies viral protein E4 E1 Supplied in trans by 293 or 911 packaging...adenovirus has two genes deleted: E1 and E3. E1 is supplied by the adenovirus packaging lines 293 or 911; ...One can also co-transform standard BJ5183 with supercoiled pAdEasy™ and the shuttle vector, but this method...capacity E4 Essential for viral transcription; Supplied by the pAdEasy-1 packaging plasmid or 911E4 packaging...

... endA Delta(sr1-recA) mcrB mrr hsdS20 (rB- mB-) supE44 ara14 galK2 lacY1 proA2 rpsL20(StrR) xyl5 lambda...lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA JM109 Addgene; Promega ...McrA-) recA1 endA1 gyrA96 thi-1 hsdR17(rK- mK+) supE44 relA1 Delta(lac- proAB) [F traDelta36 proAB lacIqZDeltaM15...Invitrogen F– mcrB mrr hsdS20 (rB–, mB–) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20 (StrR ) xyl-5 λ...

...placed between the two ITRs, and Rep and Cap are supplied in trans . In addition to Rep and Cap, AAV requires...used for research purposes, the Rep protein is supplied in trans , eliminating the ability of rAAV to ...

...compatible with truncated gRNAs to increase specificity SuperFi-Cas9 - increases fidelity with reduced nuclease...consecutive binding and degradation of negatively supercoiled invader DNA by cascade and CAS3. Molecular Cell...

... guessed from their name, pooled libraries are supplied as a mixed population of plasmids in a single ...

... above (envelope, packaging, and transfer) are supplied by three types of plasmids, which are cotransfected...

... media change and a brief incubation period, supernatant containing the virus is removed and stored or...