Showing: 1 - 20 of 150 results
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Plasmid#60954Purpose2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherryDepositorInsertKRAB-dCas9-P2A-mCherry fusion
UseCRISPR and LentiviralTags2x NLS, HA, KRAB domain, and mCherryExpressionMammalianMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP
Plasmid#60955PurposeParental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJW1504
Plasmid#62358PurposeRPS2 HA-TEV-AVI pop-in/pop-out plasmidDepositorInsertRPS2 fragment
UseYeast targetingTagsHA-TEV-AVIExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJW1505
Plasmid#62359PurposeRPL16a HA-TEV-AVI pop-in/pop-out plasmidDepositorInsertRPL16A
UseYeast taggingTagsHA-TEV-AVIExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJW1506
Plasmid#62360PurposeRPL16b HA-TEV-AVI pop-in/pop-out plasmidDepositorInsertRPL16B
UseYeast targetingTagsHA-TEV-AVIExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJW1507
Plasmid#62361PurposepFA6a -mVenus-BirA::HIS5DepositorInsertc-terminal mVenus-BirA tagging
UseYeast c-terminal tagging vectorTagsmVenus-BirAExpressionMutationL232H mutation compared to standard mVenusPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJW1508
Plasmid#62362PurposepFA6a BirA-mVenus-SSH1::HIS5DepositorInsertBirA-mVenus-Ssh1
UseTagsBirA-mVenusExpressionYeastMutationPromoterGal1AvailabilityAcademic Institutions and Nonprofits only -
pJW1509
Plasmid#62363PurposepFA6a BirA-heh2 linker-SSH1::HIS5DepositorInsertBirA-heh2-Ssh1
UseTagsExpressionYeastMutationPromoterGal1AvailabilityAcademic Institutions and Nonprofits only -
pJW1510
Plasmid#62364PurposepFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5DepositorInsertBirA-mVenus-Ubc6 tail anchor
UseTagsExpressionYeastMutationL232H mutation compared to standard mVenusPromoterSec63AvailabilityAcademic Institutions and Nonprofits only -
pJW1511
Plasmid#62365PurposepEF1a-puro-T2A-ATHmmuRPL10ADepositorInsertPuro-T2A-AVITEVHAmmuRPL10A
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
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pJW1513
Plasmid#62383PurposepRS316-pTDH3-Su9-TagBFPDepositorInsertsu9-tagBFP
UseTagsSu9 presequenceExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJW1514
Plasmid#62384PurposepCR2.1 linker-tev-linker-3X-FLAG::KanMXDepositorInserttev 3x FLAG
UseYeast c-terminal tagging vectorTagslinker-tev-linker-3xflagExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJW1515
Plasmid#62385PurposepFA6a Osm1-yoEGFP::LEU2DepositorInsertOSM1-EGFP fusion
UseTagsEGFPExpressionYeastMutationPromoterAdh1pAvailabilityAcademic Institutions and Nonprofits only -
pJW1516
Plasmid#62386PurposepFA6a Osm1(M1A)-yoEGFP::LEU2DepositorInsertOSM1-EGFP fusion
UseTagsEGFPExpressionYeastMutationMethionine 1 to AlaninePromoterAdh1pAvailabilityAcademic Institutions and Nonprofits only -
pJW1517
Plasmid#62387PurposepFA6a Osm1(M33A)-yoEGFP::LEU2DepositorInsertOSM1-EGFP fusion
UseTagsEGFPExpressionYeastMutationMethionine 33 mutated to AlaninePromoterAdh1pAvailabilityAcademic Institutions and Nonprofits only -
pJW1518
Plasmid#62388PurposepFA6a -mCherry-BirA::HIS5DepositorInsertmCherry-BirA
UseYeast c-terminal tagging vectorTagsmCherry-BirAExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJR85
Plasmid#140095PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJR89
Plasmid#140096PurposesgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.DepositorInsertsgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 150 results